半夏白术天麻汤通过FOXO1通路对血脂异常合并高血压大鼠DsbA-L/脂联素的影响  

Effects of Banxia Baizhu Tianma Decoction on DsbA-L/adiponectin in rats with dyslipidemia and hypertension through FOXO1 pathway

作　　者：王琪格 隋国媛 丁思元 裘雪莹 吕美君[1] 曹慧敏[1] 英哲铭 王群[1] 贾连群[1] 杨关林[1] WANG Qi-ge;SUI Guo-yuan;DING Si-yuan;QIU Xue-ying;LYU Mei-jun;CAO Hui-min;YING Zhe-ming;WANG Qun;JIA Lian-qun;YANG Guan-lin(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China)

机构地区：[1]辽宁中医药大学,沈阳110847

出　　处：《中华中医药杂志》2022年第5期2898-2902,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81974548);中医药传承与创新“百千万”人才工程(岐黄工程)-国家中医药领军人才支持计划(岐黄学者);国家重点研发计划“中医药现代化研究”重点专项(No.2018YFC1704400)。

摘　　要：目的:探讨半夏白术天麻汤通过FOXO1相关通路对高脂合并高血压大鼠主动脉周围脂肪组织DsbA-L/脂联素的影响。方法:采用随机数表法将48只自发性高血压大鼠(SHR)随机分为SHR组,SHR高脂组,半夏白术天麻汤低、中、高剂量组,西药组,每组8只。其中SHR组大鼠给予基础饲料,其余5组给予高脂饲料。8周后,半夏白术天麻汤低、中、高剂量组、西药组分别给予不同药物灌胃。12周后,无创血压测量分析系统仪测量各组大鼠尾动脉收缩压;测量大鼠体质量;全自动生化分析仪检测血清血脂水平;HE染色法观察主动脉PVAT病理组织学形态;ELISA法检测血清APN、TNF-α含量;Real time PCR法检测主动脉PVAT中APN、DsbA-L、FOXO1、SIRT1、C/EBP mRNA表达;免疫组化法检测主动脉PVAT中FOXO1、SIRT1、C/EBP蛋白表达。结果:与SHR组比较,SHR高脂组收缩压、体质量均显著上升(P<0.01,P<0.05);血清TG、TC、LDL-C水平均显著升高(P<0.01,P<0.05),HDL-C水平显著下降(P<0.01)。与SHR高脂组比较,半夏白术天麻汤各剂量组收缩压与体质量均显著下降(P<0.01,P<0.05);血清TG、TC、LDL-C水平均显著下降(P<0.01);HE染色示半夏白术天麻汤高剂量组改变更为明显,脂肪空泡面积减小,血清APN水平显著提高(P<0.01),TNF-α水平均显著下降(P<0.05);主动脉PVAT中APN、DsbA-L、FOXO1、SIRT1、C/EBP mRNA表达均显著提升(P<0.01,P<0.05);FOXO1、SIRT1、C/EBP蛋白表达上升。结论:半夏白术天麻汤可能通过影响FOXO1相关通路,进而调控高脂合并高血压大鼠主动脉周围脂肪组织DsbA-L/脂联素,以改善血脂与血压水平。Objective:To expore the effect of Banxia Baizhu Tianma Decoction(BBTD)on DsbA-L/Adiponectin(APN)in perivascular adipose tissue of rats with hyperlipidemia and hypertension through FOXO1 related pathway.Methods:Fortyeight SHR were randomly divided into SHR group,SHR high fat group,BBTD low-dose,medium-dose,and high-dose groups and western medicine group,with 8 rats in each group.Among them,SHR group rats were fed with basic diet,and the other five groups were fed with high-fat diet.After feeding for 8 weeks,BBTD low-,medium-and high-dose groups and western medicine group were given different drugs by gavage respectively.After 12 weeks,the systolic pressure of caudal artery was measured by noninvasive blood pressure measurement and analysis system.The body mass of rats was measured.The level of serum lipid was detected by automatic biochemical analyzer.The histopathological morphology of aortic PVAT was observed by HE staining.Serum APN and TNF-αwere detected by ELISA Content.The mRNA expressions of APN,DsbA-L,FOXO1,SIRT1 and C/EBP in aortic PVAT were detected by real time RT-PCR.The expressions of FOXO1,SIRT1 and C/EBP proteins in aortic PVAT were detected by immunohistochemistry.Results:Compared with SHR group,systolic blood pressure and body mass in SHR high fat group increased significantly(P<0.01,P<0.05),the levels of serum TG,TC and LDL-C increased significantly(P<0.01,P<0.05),and the level of HDL-C decreased significantly(P<0.01).Compared with SHR high fat group,the systolic blood pressure and body mass of BBTD each dose groups decreased significantly(P<0.01,P<0.05),the levels of serum TG,TC and LDL-C decreased significantly(P<0.01).In HE staining,the change of BBTD high-dose group was more obvious,the area of fat vacuole decreased,the level of serum APN increased significantly(P<0.01),the level of TNF-αdecreased significantly(P<0.05);The mRNA expressions of APN,DsbA-L,FOXO1,SIRT1 and C/EBP were significantly increased in aortic PVAT(P<0.01,P<0.05),and the protein expressions of FOXO1,SIRT1 and C/EBP were

关 键 词：半夏白术天麻汤 高脂 自发性高血压大鼠 主动脉周围脂肪组织 脂联素 高血压病 FOXO1通路 

分 类 号：R285.5[医药卫生—中药学]