补肾解毒方对放射致感染性肺损伤的防护作用研究  

Study of Protective Effect of Bushen Jiedu Decoction on Radiation-Induced Infectious Lung Injury

作　　者：余雷 李延晖 曹慧敏 刘冬书 刘晓辰 邹梦颖 王磊 张蓉 YU Lei;LI Yanhui;CAO Huimin;LIU Dongshu;LIU Xiaochen;ZOU Mengying;WANG Lei;ZHANG Rong

机构地区：[1]火箭军特色医学中心中医科,北京100088 [2]苏州大学药学院,江苏苏州215000

出　　处：《新中医》2022年第9期11-15,共5页New Chinese Medicine

基　　金：国家自然科学基金项目(81673873);国家自然科学基金青年项目(81703979)。

摘　　要：目的:探讨补肾解毒方对放射致感染性肺损伤的防护作用。方法:选取72只C57BL/10J小鼠,随机分为空白对照组、单纯照射组、氨磷汀组、中药组,每组18只,建立各组小鼠模型,在实验开始后第11天,空白对照组小鼠不接受照射,余各组小鼠均接受一次性6 Gy^(60)Coγ射线全身照射,其中氨磷汀组在照射前30 min内注射氨磷汀。并于照射后第1天、第7天、第14天每组随机抽取6只小鼠进行取材,计算肺脏系数,采用酶联免疫吸附实验(ELISA)检测降钙素原(PCT)、C-反应蛋白(CRP)、白细胞介素-6 (IL-6),并在光学显微镜下观察小鼠肺组织病理形态学变化。结果:照射后第1天,空白对照组肺系数水平低于余3组(P<0.05),同时单纯照射组高于氨磷汀组和中药组(P<0.05);照射后第7天,空白对照组肺系数水平低于单纯照射组和氨磷汀组(P<0.05),同时单纯照射组高于氨磷汀组和中药组(P<0.05);照射后第14天,单纯照射组肺系数水平高于余3组(P<0.05)。单纯照射组病理形态学变化最明显,氨磷汀组和中药组可明显减轻肺组织病理变化,其中中药组优于氨磷汀组。照射后第1天,空白对照组PCT、CRP、IL-6水平显著低于余3组(P<0.05),同时单纯照射组CRP、IL-6水平高于氨磷汀组与中药组(P<0.05);照射后第7天,PCT、CRP、IL-6水平中,空白对照组低于余3组(P<0.05),单纯照射组高于氨磷汀组和中药组(P<0.05),同时中药组低于氨磷汀组(P<0.05);照射后第14天,空白对照组PCT、IL-6水平低于单纯照射组和氨磷汀组(P<0.05),单纯照射组高于氨磷汀组和中药组(P<0.05),中药组低于氨磷汀组(P<0.05),单纯照射组CRP水平高于余3组(P<0.05)。结论:补肾解毒方能够减轻放射致感染性肺损伤的感染程度,降低PCT、CRP水平,发挥抗炎抗损伤作用。Objective:To explore the protective effect of Bushen Jiedu decoction on radiation-induced infectious lung injury.Methods:72 C57BL/10J mice were randomly divided into blank control group,simple irradiation group,amifostine group and traditional Chinese medicine group,with 18 mice in each group.The mouse models of each group were established.On the 11^(th) day after the beginning of the experiment,the mice in blank control group did not receive irradiation,and the other mice in each group received one-time 6 Gy^(60)Coγ30 min before the whole body irradiation with fostine.Six mice in each group were randomly selected on the 1^(st),7^(th) and 14^(th) day after irradiation.The lung coefficient was calculated.Procalcitonin(PCT),C-reactive protein(CRP) and interleukin-6(IL-6) were detected by enzyme-linked immunosorbent assay(ELISA),and the pathomorphological changes of mouse lung were observed under optical microscope.Results:On the first day after irradiation,the lung coefficient of the blank control group was lower than that of the other three groups(P<0.05),and that of the simple irradiation group was higher than that of amifostine group and traditional Chinese medicine group(P<0.05).On the 7^(th)day after irradiation,the lung coefficient level in the blank control group was lower than that in the simple irradiation group and amifostine group(P<0.05),while that in the simple irradiation group was higher than that in the amifostine group and traditional Chinese medicine group(P<0.05).On the 14^(th) day after irradiation,the lung coefficient in the simple irradiation group was higher than that in the other three groups(P<0.05).The pathomorphological changes were the most obvious in the simple irradiation group.Amifostine group and traditional Chinese medicine group could significantly reduce the pathological changes of lung tissue,and the traditional Chinese medicine group was better than amifostine group.On the first day after irradiation,the levels of PCT,CRP and IL-6 in the blank control group were significantly l

关 键 词：放射致感染性肺损伤 补肾解毒方 病理观察 降钙素原 C-反应蛋白 白细胞介素-6 

分 类 号：R285.5[医药卫生—中药学]