结核分枝杆菌/利福平耐药性实时荧光PCR检测与抗酸染色在肺结核中的诊断价值  

Diagnostic value of GeneXpert Mycobacterium tuberculosis/Rifampin technology and acid-fast staining in pulmonary tuberculosis

作　　者：李幸旻 LI Xingmin(Department of Clinical Laboratory,Sino-Singapore Guangzhou Knowledge City Hospital,Guangdong Province,Guangzhou510363,China)

机构地区：[1]广州市中新广州知识城医院检验科,广东广州510363

出　　处：《中国当代医药》2022年第14期146-149,共4页China Modern Medicine

摘　　要：目的评估并分析结核病诊断中结核分枝杆菌/利福平耐药性实时荧光PCR检测(GeneXpert MTB/RIF)技术与抗酸染色的诊断价值。方法选取2019年10月至2021年10月中新广州知识城医院收治的80例疑似肺结核患者作为研究对象,采用GeneXpert MTB/RIF技术检测、抗酸染色检测,以临床病理诊断为金标准,比较两种方法的阳性率、诊断效能。结果80例疑似肺结核患者经临床病理诊断确诊42例,GeneXpert MTB/RIFF检测阳性率高于抗酸染色阳性率;GeneXpert MTB/RIF检测诊断灵敏度、特异度、准确度分别为90.48%、100.00%、95.00%,抗酸染色的诊断灵敏度、特异度、准确度分别为33.33%、100.00%、65.00%,GeneXpert MTB/RIF技术诊断灵敏度、准确度高于抗酸染色结果,差异有统计学意义(P<0.05)。结论在肺结核诊断中应用GeneXpert MTB/RIF具有显著的诊断价值,大大提高结核分枝杆菌、利福平耐药性检测的灵敏度及特异度,为疾病临床诊疗活动开展提供有效参考。Objective To evaluate and analyze the diagnostic value of GeneXpert Mycobacterium tuberculosis/Rifampin(GeneXpert MTB/RIF)technology and acid-fast staining in the diagnosis of tuberculosis.Methods A total of 80 patients with suspected tuberculosis admitted to the Sino-Singapore Guangzhou Knowledge City Hospital from October 2019 to October 2021 were selected as the research objects.GeneXpert MTB/RIF technology,acid-fast staining were used for detection,and clinical pathological diagnosis was the gold standard.Compare the positive rate and diagnostic efficiency of the two methods.Results A total of 42 cases of 80 suspected tuberculosis patients were diagnosed by clinical pathology.The positive rate of GeneXpert MTB/RIFF technology was higher than that of acid-fast staining.The diagnostic sensitivity,specificity,and accuracy of GeneXpert MTB/RIF technology are 90.48%,100.00%and 95.00%,respectively.The diagnostic sensitivity,specificity,and accuracy of acid-fast staining are 33.33%,100.00%and 65.00%,respectively.The diagnostic sensitivity and accuracy of GeneXpert MTB/RIF technology were higher than those of acid-fast staining,and the differences were statistically significant(P<0.05).Conclusion The application of GeneXpert MTB/RIF technology in the diagnosis of tuberculosis has significant diagnostic value,greatly improving the sensitivity and specificity of the detection of Mycobacterium tuberculosis and Rifampicin resistance,and providing an effective reference for the development of clinical disease diagnosis and treatment activities.

关 键 词：结核分枝杆菌/利福平耐药性实时荧光PCR检测 抗酸染色 肺结核 诊断价值 灵敏度 特异度 

分 类 号：R655.3[医药卫生—外科学]