北极海洋来源真菌Aspergillus sp.HDN19-401次级代谢产物的研究  

Studies on secondary metabolites from the Arctic marine-derived fungus Aspergillus sp.HDN19-401

作　　者：黄璐瑶 张文壮 钱亚 吴家劲 张国建 车茜 李德海[1] 朱天骄[1] Huang Lu-yao;Zhang Wen-zhuang;Qian Ya;Wu Jia-jin;Zhang Guo-jian;Che Qian;Li De-hai;Zhu Tian-jiao(Key Laboratory of Marine Drugs,MOE,China,College of Medicine,Ocean University of China,Qingdao 266000)

机构地区：[1]海洋药物教育部重点实验室,中国海洋大学医药学院,青岛266000

出　　处：《中国抗生素杂志》2022年第5期488-493,共6页Chinese Journal of Antibiotics

基　　金：国家重点研发计划(No.2018YFC1406705);新药创制国家科技重大专项(No.2018ZX09735004)。

摘　　要：目的研究北极海洋来源真菌Aspergillus sp.HDN19-401的次级代谢产物及其生物活性。方法采用反相硅胶柱色谱、葡聚糖凝胶Sephadex LH-20、半制备HPLC等方法分离纯化该菌株的发酵粗提物,通过ESI-MS、NMR等波谱数据分析并与文献对比确定化合物的结构,最低抑菌浓度(minimal inhibitory concentration,MIC)采用微量肉汤稀释法测定,抗肿瘤活性采用MTT法测定。结果与讨论从Aspergillus sp.HDN19-401中分离得到9个化合物,通过对比核磁数据确定为:6-O-methylaverufin(1)、6,8-di-O-methylaverufin(2)、6,8-di-O-methylnidurufin(3)、aversin(4)、oxisterigmatocystin A(5)、oxisterigmatocystin B(6)、fellutamideC(7)、notoamidesB(8)、notoamidesC(9),除化合物2外,其余均为首次从极地来源真菌中分离得到,化合物7对K562细胞系具有中等的抑制活性,IC_(50)值为7.78μmol/L。Objective To study the secondary metabolites produced by marine-derived fungus Aspergillus sp.HDN19-401 isolated from the Arctic and their biological activities.Methods The fermentation extracts were isolated and purified by reversed phase silica gel column chromatography,sephadex LH-20 and semi-preparative HPLC and so on.The chemical structures of these compounds were confirmed by ESI-MS and NMR spectral analysis and comparison with the data of literature.MICs were obtained by the broth microdilution method.Antitumor activity was studied by the MTT assay.Results and Conclusion Nine compounds were isolated from the extracts of Aspergillus sp.HDN19-401,and were identified as 6-O-methylaverufin(1),6,8-di-O-methylaverufin(2),6,8-di-O-methylnidurufin(3),aversin(4),oxisterigmatocystin A(5),oxisterigmatocystin B(6),fellutamide C(7),notoamides B(8),and notoamides C(9).Except compound 2,the other compounds were isolated from Arctic fungus for the first time.Compound 7 showed moderately cytotoxic activity against K562 cell line with an IC_(50) value of 7.78μmol/L.

关 键 词：北极海洋真菌 曲霉属 次级代谢产物 生物活性 

分 类 号：R979.14[医药卫生—药品]