茯苓多糖对鸡免疫功能的影响  

Effect of Poria cocos Mushroom Polysaccharides on Immune Function of Chickens

作　　者：王萍 王玉堃 于天明 尹贻奎 李梁梁 王玉霞 许方方 谭庆强 王莹莹 李凤华 WANG Ping;WANG Yukun;YU Tianming;YIN Yikui;LI Liangliang;WANG Yuxia;XU Fangfang;TAN Qingqiang;WANG Yingying;LI Fenghua(Shandong Xundakang Veterinary Medicine Co.,Ltd.,Jinan Shandong 250300)

机构地区：[1]山东迅达康兽药有限公司,山东济南250300

出　　处：《现代农业科技》2022年第11期159-161,170,共4页Modern Agricultural Science and Technology

摘　　要：为探讨茯苓多糖对海兰褐公雏鸡体内外免疫活性的影响,将120羽1日龄海兰褐公雏鸡随机分成6组,分别为空白对照组、模型组、免疫对照组(1 kg饲料添加0.2 g盐酸左旋咪唑)、茯苓多糖高剂量组(1 kg饲料添加2.0 g茯苓多糖)、茯苓多糖中剂量组(1 kg饲料添加1.0 g茯苓多糖)、茯苓多糖低剂量组(1 kg饲料添加0.5 g茯苓多糖),用MTT法检测不同浓度茯苓多糖对鸡外周血淋巴细胞、脾脏细胞增殖的影响,ELISA法测定血清中细胞因子IL-2和IFN-γ含量,屠杀、称重、计算各组鸡脾脏指数、胸腺指数、法氏囊指数。结果表明,茯苓多糖对鸡外周血淋巴细胞、脾脏细胞增殖具有刺激作用,浓度越高,刺激作用越强,呈现剂量依赖性;茯苓多糖高、中、低剂量组鸡血清中IL-2和IFN-γ均高于模型组,茯苓多糖各组免疫器官指数高于模型组。这表明茯苓多糖能提高海兰褐公雏鸡的免疫功能。In order to investigate the effect of Poria cocos mushroom polysaccharides on the immune activity of Hy-line brown male chicks in vivo and in vitro,120 one-day-old Hy-line brown male chicks were randomly divided into 6 groups,namely the blank control group,the model group,the immune control group(adding 0.2 g levamisole hydrochloride to 1 kg feed),and the Poria cocos mushroom polysaccharides high-dose group(adding 2.0 g Poria cocos mushroom polysaccharides to 1 kg feed),the Poria cocos mushroom polysaccharides medium dose group(adding 1.0 g Poria cocos mushroom polysaccharides to 1 kg feed),and the Poria cocos mushroom polysaccharides low-dose group(adding 0.5 g Poria cocos mushroom polysaccharides to 1 kg feed).The effects of different concentrations of Poria cocos mushroom polysaccharides on the proliferation of chicken peripheral blood lymphocytes and spleen cells was determined by MTT.ELISA method was used to determine the content of cytokines(IL-2 and IFN-γ)in serum.The chickens were slaughtered and weighed,and then the spleen index,thymus index,and bursa index of each group of these chickens were calculated.The results showed that Poria cocos mushroom polysaccharides stimulated the proliferation of peripheral blood lymphocytes and spleen cells of the chickens.The higher the concentration was,the stronger the stimulating effect was achieved,which was dose-dependent.The levels of IL-2 and IFN-γin the serum of chickens with the high,medium and low-dose of Poria cocos mushroom polysaccharides were higher than those in the model group.The immune organ index of each group of Poria cocos mushroom polysaccharides was higher than that of the model group.This indicated that Poria cocos mushroom polysaccharides could improve the immune function of Hy-line brown male chicks.

关 键 词：茯苓多糖 免疫活性 海兰褐公雏鸡 

分 类 号：S831[农业科学—畜牧学]