隔药饼灸对动脉粥样硬化易损斑块兔腹主动脉TNF-α、p38MAPK及eNOS蛋白表达的影响  

Effects of Herbal-cake-separated Moxibustion on Expressions of Abdominal Aorta of TNF-α,p38MAPK and eNOS in Rabbits with Atherosclerotic Vulnerable Plaque

作　　者：宋家薇 饶泽华 廖意娟 马逸杰 吴雪芬[1] 刘欣 易丽贞 岳增辉[1] SONG Jiawei;RAO Zehua;LIAO Yijuan;MA Yijie;WU Xuefen;LIU Xin;YI Lizhen;YUE Zenghui(College of Acupuncture&Moxibustion and Tui-na,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学针灸推拿学院,湖南长沙410208

出　　处：《中国中医药信息杂志》2022年第6期81-86,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81874430);湖南省研究生科研创新重点项目(CX20200753);湖南省研究生科研创新项目(CX20200778、CX20200779)。

摘　　要：目的观察隔药饼灸对动脉粥样硬化(AS)易损斑块兔肿瘤坏死因子-α(TNF-α)、p38丝裂原活化蛋白激酶(p38MAPK)、内皮型一氧化氮合酶(eNOS)表达的影响,探讨其稳定AS易损斑块的作用机制。方法将58只普通级新西兰兔随机分为空白组、模型组、直接灸组、隔药饼灸组和西药组。通过高脂饲料喂养+腹主动脉球囊损伤术+基因转染+触发斑块破裂建立AS易损斑块兔模型。直接灸组和隔药饼灸组分别直接灸及隔药饼灸“巨阙”、双侧“天枢”“丰隆”或双侧“心俞”“肝俞”“脾俞”,每日1次,连续8周。ELISA检测兔血清载脂蛋白A(apo-A)、载脂蛋白B(apo-B)含量,透射电镜观察腹主动脉超微结构,免疫组化法检测腹主动脉组织TNF-α、p38MAPK、eNOS蛋白表达。结果与空白组比较,模型组兔血清apo-A含量显著减少(P<0.01),apo-B含量显著增加(P<0.01),腹主动脉血管壁严重破坏,有大量泡沫细胞聚集和脂滴沉积,TNF-α、p38MAPK蛋白表达显著升高(Ρ<0.01),eNOS蛋白表达显著降低(Ρ<0.01);与模型组比较,直接灸组、隔药饼灸组和西药组兔血清apo-A含量显著增加(P<0.05,P<0.01),apo-B含量显著减少(P<0.05,P<0.01),直接灸组兔腹主动脉内皮细胞损伤,脂滴散在分布于泡沫池中,隔药饼灸组及西药组兔腹主动脉内皮细胞损伤程度较轻,各干预组兔腹主动脉组织TNF-α、p38MAPK蛋白表达均显著降低(Ρ<0.05,Ρ<0.01),eNOS蛋白表达显著升高(Ρ<0.05,Ρ<0.01);与直接灸组比较,隔药饼灸组兔血清apo-B含量显减少(P<0.01),腹主动脉组织TNF-α蛋白表达显著降低(Ρ<0.05),eNOS蛋白表达显著升高(Ρ<0.05,Ρ<0.01);与西药组比较,隔药饼灸组兔血清apo-B含量明显减少(P<0.05)。结论隔药饼灸可能通过抑制TNF-α、p38MAPK蛋白表达,促进eNOS蛋白表达,保护血管内皮功能,稳定AS易损斑块。Objective To observe the effects of herbal-cake-separated moxibustion on the expressions of tumor necrosis factor-α(TNF-α),p38 mitogen-activated proteinkinase(p38MAPK),endothelial nitric oxide synthase(eNOS)in rabbits with atherosclerotic vulnerable;To explore its mechanism of stabilizing atherosclerotic vulnerable plaque.Methods Totally 58 ordinary New Zealand rabbits were randomly divided into blank group,model group,direct moxibustion group,herbal-cake-separated moxibustion group and Western medicine group.The atherosclerotic vulnerable plaque model was established by high-fat forage feeding+balloominduced abdominal aorta injury+gene transfection+trigger plaque rupture.Direct moxibustion group and herbal-cake-separated moxibustion group were applied to“Juque”(RN 14)and bilateral“Tianshu”(ST 25),“Fenglong”(ST 40),or bilateral“Xinshu”(BL 15),“Ganshu”(BL 18)and“Pishu”(BL 20),once daily,for 8 weeks.The contents of apolipoprotein A(apo-A)and apolipoprotein B(apo-B)in serum were detected by the ELISA.Ultrastructure of the abdominal aorta was observed by transmission electron microscopy.Immunohistochemistry was used to detect the expression of TNF-α,p38MAPK,eNOS protein in abdominal aortic tissue.Results Compared with the blank group,the content of apo-A in serum of the rabbits in model group significantly reduced(P<0.01),the content of apo-B significantly increased(P<0.01).The abdominal aorta was severely damaged,with a large number of foam cells gathered and lipid droplets deposited.The expressions of TNF-αand p38MAPK protein in abdominal aortic tissue significantly increased(P<0.01),the expression of eNOS protein significantly decreased(P<0.01).Compared with the model group,the content of apo-A in serum of the rabbit in direct moxibustion group,herbal-cake-separated moxibustion group and Western medicine group increased significantly(P<0.05,P<0.01),the content of apo-B significantly decreased(P<0.05,P<0.01).The abdominal aortic endothelial cells of the rabbits in direct moxibustion grou

关 键 词：隔药饼灸 动脉粥样硬化 易损斑块 血管内皮功能 肿瘤坏死因子-α P38丝裂原活化蛋白激酶 内皮型一氧化氮合酶 兔 

分 类 号：R245.8[医药卫生—针灸推拿学]