多粘类芽孢杆菌LY1的全基因组测序及分析  

Whole-Genome Sequencing and Analysis on Paenibacillus polymyxa LY1

作　　者：聂俊辉 王平 张立新 王通 郭建军[1] 曾静[1] 袁林[1] NIE Jun-hui;WANG Ping;ZHANG Li-xin;WANG Tong;GUO Jian-jun;ZENG Jing;YUAN Lin(Institute of Microbiology,Jiangxi Academy of Sciences,Nanchang 330096,PRC;Agriculture,Forestry and Water Science Research Center of Shangrao,Shangrao 334000,PRC)

机构地区：[1]江西省科学院微生物研究所,江西南昌330096 [2]上饶市农林水科学研究中心,江西上饶334000

出　　处：《湖南农业科学》2022年第5期1-6,共6页Hunan Agricultural Sciences

基　　金：江西省科学院科研开发专项基金博士项目(2020-YYB-08);江西省科学院重点科研开发项目(2020-YZD21);江西省科学院省级科技计划项目包干制试点示范项目重大产业技术攻关项目(2021YSBG10005);国家自然科学基金(32060772);江西省重点研发计划(20202BBF63018,20212BBF63022)。

摘　　要：为全面了解多粘类芽孢杆菌LY1的遗传背景,利用基因组测序技术全面解析LY1菌株的基因组序列,再通过生物信息学的方法比较不同菌株基因间的差异性和相似性,获得LY1菌株的基因组基本特征、基因功能注释及分类、系统发育进化关系等关键信息,从全基因组水平了解其系统发育进化关系。结果表明:多粘类芽孢杆菌LY1 的基因组长度为5 765 474 bp,共有6 086个蛋白质编码基因、162个 tRNA 基因、42个rRNA 基因,GC含量约为45.23%;在GO功能注释中,在生物过程分类下代谢过程和细胞过程中的基因占比最多,分别占比40.77%和38.98%;在细胞组分分类下,分类到细胞和细胞组成中的基因占比最多,为35.18%;在分子功能分类下,分类到催化活性和结合中的基因占比最多,分别为40.34%和35.78%;系统发育进化分析显示,菌株LY1与Paenibacillus polymyxa M1、Paenibacillus polymyxa OSY-DF、Paenibacillus polymyxa SC2、Paenibacillus polymyxa E681等菌株进化距离较近。In order to study the genome structure and function of Paenibacillus polymyxa LY1,whole-genome sequencing technology was used for sequencing and analysis.The results showed that the genome of Paenibacillus polymyxa (P.p) LY1 contained 5,765,474bp,6,086 protein-coding genes,162 tRNA genes,and 42 rRNA genes,and the GC content was about 45.23%.GO function annotations showed that genes in metabolic processes and cellular processes occupied the largest proportion under the biological process classification,accounting for 40.77% and 38.98% respectively;under the classification of cell components,the proportion of genes classified into cell and cell part was the largest,at 35.18%;under the molecular function classification,the genes classified into catalytic activity and binding accounted for the largest proportion,40.34% and 35.78%,respectively.Phylogenetic evolutionary analysis showed that P.p LY1 was close to P.p M1,P.p OSY-DF,P.p SC2,and P.p E681.In this study,the basic genome-wide characteristics of P.p LY1 were obtained,which provided a basis for subsequent genetic study of Paenibacillus polymyxa.

关 键 词：多粘类芽孢杆菌 基因组 测序 基因功能 

分 类 号：Q933[生物学—微生物学]