牦牛妊娠期和产后乏情期卵巢蛋白组学分析  

Ovarian Proteomics of Yaks in Pregnancy and Postpartum Anestrus

作　　者：麻俊渊 王进娥 李洋 胡凇茗 陈舟 李书元 杨妍梅[1] 扎西英派[1] 霍生东[1] MA Junyuan;WANG Jine;LI Yang;HU Songming;CHEN Zhou;LI Shuyuan;YANG Yanmei;ZHAXI Yingpai;HUO Shengdong(Life Science and Engineering College,Northwest University for Nationalities,Lanzhou,Gansu 730030,China)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730030

出　　处：《福建农业学报》2022年第4期429-438,共10页Fujian Journal of Agricultural Sciences

基　　金：中央高校基本科研业务费专项资金项目(31920190204、31920200076);甘肃省重点研发计划项目(17YF1NA067);甘肃省教育厅优秀研究生“创新之星”项目(2021CXZX-683)。

摘　　要：【目的】研究牦牛产后乏情期和妊娠期卵巢中差异蛋白的表达情况。【方法】选择青海省湟源县一户牧民自然放牧牛群中处于妊娠期和产后乏情期牦牛各3头为研究对象,采集卵巢组织,利用TMT定量蛋白质组学技术发掘不同时期卵巢差异表达蛋白。【结果】妊娠期和产后乏情期卵巢组织中差异表达蛋白有57个,其中乏情期卵巢组织中上调蛋白38个,下调蛋白19个;COG分析发现,差异表达蛋白功能集中于能量的生产与转化、蛋白质周转、氨基酸转运与代谢等过程;GO分析发现,差异表达蛋白集中于代谢、繁殖等生物过程,涉及细胞器成分等细胞组分,参与转运活性等分子功能;KEGG分析发现,差异表达蛋白共参与27条信号通路,其中,缓激肽(BK)、胆固醇侧链裂解酶(P450scc)参与卵巢类固醇激素生成通路,谷胱甘肽过氧化物酶1(GSH-P1)、谷胱甘肽S转移酶A2(GSTA2)、谷胱甘肽转移酶(GST)和天冬氨酸转氨酶(AST)参与氨基酸转运与蛋白质代谢通路,磷脂酰肌醇-3-激酶蛋白(PI3K)参与PI3K-Akt细胞凋亡信号通路。【结论】BK、P450scc、GSH-P1、GSTA2、GST、AST和PI3K与牦牛乏情的产生密切相关。【Objective】 Differentially expressed proteins(DEPs) in ovaries of yak s in pregnancy and postpartum anestrus were identified. 【Method】 Ovarian tissues of 3 randomly selected yaks each in pregnancy or postpartum anestrus from a natural grazing herd in Huangyuan County, Qinghai Province were sampled for DEP identification by the TMT method.【Result】 Thirty-eight upregulated and 19 downregulated DEPs were found in the yaks. The COG analysis indicated the major functions of the proteins related to the production and transformation of energy, protein turnover, and amino acid transport and metabolism. The Go analysis showed that they were mainly annotated in the biological processes of metabolism and reproduction, associated with the cellular component including organelle cell components, and participated in molecular function including the molecular transport activity. Whereas KEGG revealed their involvements in 27 signaling pathways that included BK and P450scc in the ovarian steroid hormone production, GSH-P1, GSTA2, GST, and AST in the amino acid transport and protein metabolism, and PI3K in the PI3K-Akt apoptosis signaling. 【Conclusion】 The current study identified specific DEPs in yak ovary, i.e., BK, P450scc, GSH-P1, GSTA2, GST, AST, and PI3K, that were closely related to the onset of anestrus in the animal.

关 键 词：妊娠期 产后乏情 蛋白质组学 卵巢 牦牛 

分 类 号：S857.21[农业科学—临床兽医学]