清火胶囊对发热大鼠的解热作用及其机制研究  

Antipyretic Effect and Mechanism of Qinghuo Capsules on Lipopolysaccharide-induced Fever in Rats

作　　者：郑洋滨 罗跃华 刘宁 艾力奇 吴良发 Zheng Yangbin;Luo Yuehua;Liu Ning;Ai Liqi;Wu Liangfa(Jiangxi Institute for Drug Control,NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine,Jiangxi Provincial Engineering Research Center of Drug and Medical Device Quality,Nanchang 330029,China;First Affiliated Hospital of Nanchang University)

机构地区：[1]江西省药品检验检测研究院,国家药品监督管理局中成药质量评价重点实验室,江西省药品与医疗器械质量工程技术研究中心,南昌330029 [2]南昌大学第一附属医院

出　　处：《中国药师》2022年第5期816-819,共4页China Pharmacist

基　　金：江西省重点研发计划项目(编号:20203BBGL73215)。

摘　　要：目的:探究清火胶囊对脂多糖所致大鼠发热模型的解热作用,并研究其作用机制。方法:60只SPF级SD健康大鼠按基础体温随机分为6组:正常对照组、模型组、阳性对照组(阿司匹林,0.125 g·kg^(-1))、清火胶囊高(1 g·kg^(-1))、中(0.5 g·kg^(-1))、低(0.25 g·kg^(-1))剂量组,每组10只。连续给药7 d,末次给药30 min后给予20μg·kg^(-1)脂多糖溶液造模,记录造模后4 h内的大鼠体温变化,取大鼠血清测定血清白细胞介素⁃6(IL⁃6)、白细胞介素⁃1β(IL⁃1β)、肿瘤坏死因子⁃α(TNF⁃α)含量,取大鼠下丘脑测定环磷酸腺苷(cAMP)、下丘脑前列腺素E_(2)(PGE_(2))以及下丘脑腹中隔区精氨酸加压素(AVP)水平。结果:造模后,清火胶囊高、中剂量组在造模后3、4 h的体温升高幅度低于模型组,低剂量组在4 h的体温升高幅度低于模型组(P<0.05);清火胶囊高剂量组TNF⁃α、IL⁃6、IL⁃1β水平均低于模型组,清火胶囊中剂量组TNF⁃α、IL⁃1β水平均低于模型组,清火胶囊低剂量组TNF⁃α水平均低于模型组(P<0.05)。清火胶囊各剂量组大鼠cAMP、PGE2水平均低于模型组(P<0.05),AVP水平与模型组比较差异无统计学意义(P>0.05)。结论:清火胶囊能够降低发热大鼠体温,抗炎效果明显,其解热功效可能与降低各种炎症因子水平,抑制下丘脑发热介质PGE2与cAMP有关。Objective:To explore the antipyretic effect of Qinghuo capsules on lipopolysaccharide induced fever in rats and its action mechanism.Methods:Sixty SPF SD healthy rats were randomly divided into six groups according to the basal body temperature:normal control group,model group,positive control group(aspirin,0.125 g·kg^(-1)),and high(1 g·kg^(-1)),medium(0.5 g·kg^(-1))and low(0.25 g·kg^(-1))dosage groups of Qinghuo capsules with 10 rats in each group.The treatment course was 7 days,and 20μg·kg^(-1) lipopolysaccharide solution was given 30 minutes after the last drug administration to make fever model.The changes of body temperature within 4 hours after modeling were observed,and the serum levels of interleukin-6(IL-6),interleukin^(-1)β(IL^(-1)β)and tumor necrosis factor-α(TNF-α),as well as cyclic adenosine monophosphate(cAMP),hypothalamic prostaglandin E_(2)(PGE_(2))and arginine vasopressin(AVP)in abdominal septum of hypothalamus were compared at the highest point of body temperature in each group.Results:After modeling,the increase of body temperature at 3 and 4 hours after modeling of high and medium doses of Qinghuo capsules groups was lower than that of the model group,and the increase of body temperature at 4 hours after modeling of low dose group was lower than that of the model group,and the difference had statistical significance(P<0.05).The levels of TNF-α,IL-6 and IL^(-1)βin the high dose group of Qinghuo capsules were lower than those in the model group,the levels of TNF-αand IL^(-1)βin the medium dose group were lower than those in the model group,and the level of TNF-αin the low dose group was lower than that in the model group,and the differences were statistically significant(P<0.05).The levels of cAMP and PGE2 in each dose group of Qinghuo capsules were lower than those in the model group,and the difference was statistically significant(P<0.05).There was no significant difference in the AVP level between the Qinghuo capsules groups and the model group(P>0.05).Conclusion:Qinghuo capsul

关 键 词：清火胶囊 脂多糖 体温 炎性因子 体温调节介质 

分 类 号：R285.5[医药卫生—中药学]