力学环境调控骨基质仿生矿化  

Biomechanical Environment Regulates Biomimetic Mineralization of Bone Matrix

作　　者：马春阳 杜田明 牛旭锋 樊瑜波 MA Chunyang;DU Tianming;NIU Xufeng;FAN Yubo(Beijing Advanced Innovation Center for Biomedical Engineering,Key Laboratory of Biomechanics and Mechanobiology,Ministry of Education,Beijing Key Laboratory for Optimal Design,Test&Evaluation Technology of High-End Medical Implants and Interventional Instruments,Institute for Medicine and Engineering Interdisciplinary Innovation,School of Medical Science and Engineering,School of Biological Science and Medical Engineering,Beihang University,Beijing 100091,China;Beijing International Science and Technology Cooperation Base for Intelligent Physiological Measurement and Clinical Transformation,Department of Biomedical Engineering,Faculty of Environment and Life,Beijing University of Technology,Beijing 100124,China)

机构地区：[1]北京市生物医学工程高精尖创新中心,生物力学与力学生物学教育部重点实验室,高端植介入医疗器械优化设计与评测技术北京市重点实验室,北京航空航天大学医工交叉创新研究院,医学科学与工程学院,生物与医学工程学院,北京100091 [2]北京工业大学环境与生命学部,生物医学工程系,智能化生理测量与临床转化北京市国际科技合作基地,北京100124

出　　处：《医用生物力学》2022年第2期200-210,共11页Journal of Medical Biomechanics

基　　金：国家自然科学基金项目(11872097,11827803,U20A20390,11272038);国家重点研发计划(2020YFC0122204);高等学校学科创新引智计划(B13003)。

摘　　要：骨缺损一直以来都是威胁人类生命健康的重要原因,人工仿生骨修复替代材料是目前治疗骨损伤最为有效、可行的解决途径之一。要研发人工骨仿生材料,必先构建体外仿生矿化体系,以研究天然骨基质的矿化机制。胶原是矿化发生的模板,其交联度、直径、渗透压和表面电荷等性质会直接影响矿化的进行。矿化发生的生化和力学环境对矿化过程的影响也十分明显,特别是非胶原蛋白和流体切应力。流体切应力是骨组织在微观环境下受到的最主要力学刺激方式,对骨骼生长、修复以及健康维护都具有重要意义。不同水平和加载方式的切应力对无定形磷酸钙向骨磷灰石的转化、胶原纤维的自组装和定向排列以及分层纤维内矿化的形成具有显著作用。本文总结影响骨基质矿化的因素及其作用机制,重点介绍流体切应力对胶原矿化的调控作用,并展望未来的发展方向。Bone defects have always been an important cause of threat to human health,and artificial biomimetic bone repair replacement materials are currently one of the most effective and feasible solution approaches to treat bone damage.To develop artificial bone biomimetic materials,an in vitro biomimetic mineralization system must be constructed first to study in vitro biomimetic mineralization mechanism of natural bone matrix.Collagen is a template for mineralization,and its properties such as crosslinking degree,diameter,osmotic pressure,and surface charge can all directly affect mineralization progress.The biochemical and mechanical environments in which mineralization occurs are also quite distinct in their effects on mineralization process,particularly noncollagenous proteins and fluid shear stress(FSS).FSS is considered to be the main mechanical stimulation of bone tissues in micro-environment,which is of great significance to bone growth,repair and health maintenance.FSS at different levels and loading regimes has significant effects on transformation of amorphous calcium phosphate to bone apatite,self-assembly and directional alignment of collagen fibrils,and formation of hierarchical intrafibrillar mineralization.In this paper,the factors affecting collagen mineralization and their mechanism were summarized,with focus on regulation of FSS on collagen mineralization,and development direction in future was also prospected.

关 键 词：骨基质 仿生矿化 胶原 流体切应力 骨修复材料 

分 类 号：R318.01[医药卫生—生物医学工程]