可折叠微板测量单细胞三维状态下的牵张力  

Single Cell Traction Force Measured by Foldable Microplates

作　　者：赵丽君 王晨艳 张全有[2] 黄棣[1,2] 侯晋川 陈维毅[1,2] ZHAO Lijun;WANG Chenyan;ZHANG Quanyou;HUANG Di;HOU Jinchuan;CHEN Weiyi(Shanxi Key Laboratory of Materials Strength&Structural Impact,College of Biomedical Engineering,Taiyuan University of Technology,Taiyuan 030024,China;Research Center for Nanobiomaterials and Regenerative Medicine,Institute of Biomedical Engineering,College of Biomedical Engineering,Taiyuan University of Technology,Taiyuan 030024,China;College of Mathematics,Taiyuan University of Technology,Taiyuan 030024,China)

机构地区：[1]太原理工大学生物医学工程研究所材料强度与结构冲击山西省重点实验室,太原030024 [2]太原理工大学生物医学工程研究所纳米生物材料与再生医学研究中心,太原030024 [3]太原理工大学数学学院,太原030024

出　　处：《医用生物力学》2022年第2期287-291,共5页Journal of Medical Biomechanics

基　　金：国家自然科学基金项目(11572213,11632013,11502158);山西省重点研发国际合作项目(201803D421060)。

摘　　要：目的构建可用于单细胞培养的可折叠微板,建立微板折叠的有限元模型,计算单细胞三维收缩时的牵张力。方法测量细胞牵张力引起微板折叠的角度,利用有限元模拟得到弯矩与折叠角之间的关系以及牵张力与弯矩之间的关系,从而实现表征单细胞三维状态下的牵张力。结果HSF和MC3T3-E1两种类型细胞在三维状态下的单细胞折叠角度范围分别在73°~173°和49°~138°,由此测算出的细胞牵张力范围分别在55~210 nN和52~161 nN。结论构建单细胞培养的可折叠微板表征单细胞在三维结构中牵张力的方法,对进一步发展三维状态下细胞黏附、迁移和铺展中牵张力的计算具有一定参考价值。Objective To fabricate a foldable microplate for single cell culture and establish finite element model of the folding microplate,so as to calculate traction force of single cells during contraction in three-dimensional(3D)state.Methods The folding angle of the microplate casued by cell traction force was calculated.Then the relation between bending moment and folding angle as well as the relation between traction force and bending moment were derived by using finite element simulation,so as to realize the characterization of traction force for singel cell in 3D state.Results The folding angles of the microplate with HSF and MC3T3-E1 cells in 3D state were 73°-173°and 49°-138°,respectively.The single cell traction forces of HSF and MC3T3-E1 cells were 55-210 nN and 52-161 nN,respectively.Conclusions The proposed method for measuring traction force of single cells in 3D state by fabricating the foldable microplate for single cell culture will provide some references for further development of calculating traction forces in 3D cell adhesion,spreading and migration.

关 键 词：细胞牵张力 单细胞培养 可折叠微板 弯矩 

分 类 号：R318.01[医药卫生—生物医学工程]