黄芪甲苷对食管鳞癌细胞凋亡的影响  

Effect of Astragaloside Ⅳ on apoptosis of esophageal squamous cell carcinoma

作　　者：何琳莉[1,2] 黄一凡[1,2] HE Lin-li;HUANG Yi-fan(Pathological Staff Room,North Sichuan Medical College,Nanchong 637000,Sichuan Province,China;Department of Pathology,Affiliated,Hospital of North Sichuan Medical College,Nanchong 637000,Sichuan Province,China)

机构地区：[1]川北医学院病理教研室,四川南充637000 [2]川北医学院附属医院病理科,四川南充637000

出　　处：《中国临床药理学杂志》2022年第9期908-912,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的 研究黄芪甲苷对食管鳞癌细胞凋亡的影响及其调控机制。方法 体外培养Eca109细胞,用不同浓度(0、20、60和120μmol·L^(-1))的黄芪甲苷处理细胞,并把细胞随机分为4组:Control组、AST组(120μmol·L^(-1)黄芪甲苷处理)、AST+pcDNA-NC组(120μmol·L^(-1)黄芪甲苷处理+转染pcDNA-NC)、AST+pcDNA-SATB1组(120μmol·L^(-1)黄芪甲苷处理+转染pcDNA-SATB1)。用细胞计数法-8(CCK-8)检测各组细胞增殖情况,用流式细胞术检测各组细胞凋亡情况;用蛋白质印迹法检测各组细胞SATB1、Bcl-2、Bax和Cl-caspase-3蛋白表达量。结果 Control组、AST组、AST+pcDNA-NC组、AST+pcDNA-SATB1组的SATB1的蛋白表达量分别为0.89±0.11,0.34±0.06,0.38±0.06,0.76±0.09,72 h OD值分别为1.16±0.14,0.57±0.08,0.64±0.08,0.89±0.11,细胞凋亡率分别为(5.24±0.67)%,(18.99±2.03)%,(20.13±2.16)%,(7.14±0.94)%,Bcl-2蛋白表达量分别为0.94±0.10,0.39±0.05,0.42±0.06,0.81±0.09,Bax蛋白表达量分别为0.26±0.04,0.81±0.10,0.78±0.09,0.45±0.06,Cl-caspase-3蛋白表达量分别为0.27±0.05,0.95±0.11,0.91±0.13,0.46±0.07。以上指标,20、60和120μmol·L^(-1)黄芪甲苷组与0μmol·L^(-1)黄芪甲苷组相比差异均有统计学意义(均P<0.05),AST组与Control组相比,差异均有统计学意义(均P<0.05),AST+pcDNA-SATB1组与AST+pcDNA-NC组相比,差异均有统计学意义(均P<0.05)。结论 黄芪甲苷可通过下调核基质结合区结合蛋白1(SATB1)的表达抑制Eca109细胞增殖,并促进细胞凋亡。Objective To investigate the effect of Astragaloside Ⅳ on esophageal squamous cell carcinoma apoptosis and its regulatory mechanism.Methods ECA109 cells were cultured in vitro and treated with different concentrations (0, 20, 60 and 120 mol · L^(-1)) of Astragaloside Ⅳ, the cells were randomly divided into four groups:Control group (blank control ) , AST group (120 μmol · L^(-1)Astragaloside Ⅳ treatment ) , AST + pc DNA-NC group (120μmol·L^(-1)Astragaloside Ⅳ treatment + transfected pc DNA-NC ) ,AST + pc DNA-SATB1 group (120 μmol · L^(-1)Astragaloside Ⅳtreatment + pc DNA-SATB1 transfection) .Cell counting kit-8 (CCK-8) was used to detect cell proliferation,and flow cytometry was used to detect cell apoptosis in each group.The protein expressions of DNA binding protein (SATB1 ) ,B-cell lymphoma-2 (Bcl-2 ) ,Bcl-2associated X protein (Bax) and Cl-caspase-3 in each group were detected by Western blotting.Results The protein expression level of SATB1 in control group,AST group,AST + pc DNA-NC group and AST + pc DNA-SATB1group were 0.89 ± 0.11,0.34 ± 0.06,0.38 ± 0.06 and 0.76 ± 0.09,respectively,The OD value for 72 h were 1.16± 0.14,0.57 ± 0.08,0.64 ± 0.08,0.89 ± 0.11,the apoptosis rates were (5.24 ± 0.67 ) % ,(18.99 ± 2.03 ) % ,(20.13 ± 2.16) % , (7.14 ± 0.94 ) % , Bcl-2 protein expression levels were 0.94 ± 0.10,0.39 ± 0.05,0.42 ± 0.06,0.81 ± 0.09,the protein expression levels of Bax were 0.26 ± 0.04,0.81 ± 0.10,0.78 ± 0.09 and0.45 ± 0.06,the protein expression of Cl-caspase-3 were 0.27 ± 0.05,0.95 ± 0.11,0.91 ± 0.13 and 0.46 ± 0.07,respectively.As for the above indexes,there were statistically significant differences between 20,60,120 μmol·L^(-1)Astragaloside Ⅳ groups and 0 μmol·L^(-1)Astragaloside Ⅳ group (all P<0.05 );there were statistically significant differences between AST group and control group (all P<0.05);there were statistically significant differences between AST + pc DNA-SATB1 group and AST + pc DNA-NC group (all P<0.05 ) .Conclusion Astragaloside Ⅳ ca

关 键 词：黄芪甲苷 核基质结合区结合蛋白1 食管鳞癌细胞 凋亡 

分 类 号：R28[医药卫生—中药学]