长链非编码RNA PCGEM1通过转化生长因子β2/果蝇母源抗皮肤生长因子蛋白2信号通路调控膀胱尿路上皮癌细胞侵袭和转移的研究  

Long non coding RNA PCGEM1 regulates bladder urothelial cancer cells invasion and metastasis through transforming growth factor β2 and drosophila mothers against decapentaplegic 2 signaling pathway

作　　者：王华礼[1] 李刚[2] 王勇超 WANG Hua-li;LI Gang;WANG Yong-chao(Department of Urology,Xinxiang Central Hospital,Xinxiang 453000,Henan Province,China;Department of Urology,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan Province,China)

机构地区：[1]新乡市中心医院泌尿外科,河南新乡453000 [2]郑州大学第一附属医院泌尿外科,河南郑州450052

出　　处：《中国临床药理学杂志》2022年第9期924-928,933,共6页The Chinese Journal of Clinical Pharmacology

摘　　要：目的 探讨长链非编码RNA PCGEM1(LncRNA PCGEM1)通过转化生长因子β2/果蝇母源抗皮肤生长因子蛋白2(TGF-β2/Smad2)信号通路调控膀胱尿路上皮癌(BUCC)细胞侵袭和转移。方法 将BUCC细胞分为空白对照组、T24-siNC组和T24-siPCGEM1组。空白对照组置于10%胎牛血清的DMEM培养基中进行培养;T24-siPCGEM1组和T24-siNC组分别用Lipofectamine 2000将10μmol·L^(-1)的siPCGEM1和阴性对照siNC转染至细胞,然后置于10%胎牛血清的DMEM培养基中进行培养。用实时荧光定量聚合酶链反应检测LncRNA PCGEM1的表达水平,用平板克隆实验检测细胞增殖情况,用蛋白质印迹法检测TGF-β2和Smad2的表达情况。结果 T24-siPCGEM1组、T24-siNC组和空白对照组的细胞克隆数分别为(192.83±15.59),(419.00±52.15)和(432.83±57.74)个,TGF-β2相对表达量分别为0.08±0.01,0.39±0.05和0.41±0.05,Smad 2相对表达量分别为0.10±0.03,0.58±0.03和0.59±0.03,T24-siPCGEM1组的上述指标与空白对照组和T24-siNC组相比,差异均有统计学意义(均P<0.05)。结论 LncRNA PCGEM1可能通过激活TGF-β2/Smad2信号通路,从而促进BUCC的进展。Objective To investigate the regulation of long chain non-coding RNA PCGEM1(LncRNA PCGEM1) on the invasion and metastasis of bladder urothelial carcinoma(BUCC) cells through transforming growth factor β2/drosophila mothers against decapentaplegic 2(TGF-β2/Smad2). Methods BUCC cells were divided into blank control group, T24-siNC group and T24-siPCGEM1 group. The blank control group was cultured in DMEM medium containing 10% fetal bovine serum.The cells of T24-siPCGEM1 group and T24-siNC group were transfected with 10 μmol·L^(-1)siPCGEM1 and negative control siNC using Lipofectamine 2000, respectively, and then cultured in DMEM medium with 10% fetal bovine serum.The expression of LncRNA PCGEM1 was detected by real-time fluorescence quantitative polymerase chain reaction,cell proliferation was detected by plate cloning assay,and the expression of TGF-β2and Smad2 was detected by Western blot. Results The number of clones in T24-si PCGEM1 group,T24-si NC group and blank control group was(192. 83±15. 59),(419. 00±52. 15) and(432. 83±57. 74),the relative expression levels of TGF-β2 were 0. 08±0. 01,0. 39±0. 05 and 0. 41±0. 05,and the relative expression levels of Smad 2 were 0. 10±0. 03,0. 58±0. 03 and 0. 59±0. 03,respectively. The above indexes in T24-si PCGEM1 group were significantly different from those in blank control group and T24-si NC group(all P < 0. 05). Conclusion LncRNA PCGEM1 may promote the progression of BUCC by activating TGF-β2/Smad2 signaling pathway.

关 键 词：长链非编码RNA 转化生长因子β2/果蝇母源抗皮肤生长因子蛋白2 膀胱尿路上皮癌 侵袭 

分 类 号：K97[历史地理—人文地理学]