黄芪多糖对荷腹水瘤小鼠氧化应激功能的影响  

Effect of Astragalus Polysaccharides on oxidative stress in mice bearing ascites tumor

作　　者：邢家玮 陈红 余占海[1,2,3] 张宝平[1,3] XING Jia-wei;CHEN Hong;YU Zhan-hai;ZHANG Bao-ping(School of Stomatology,Lanzhou University,Lanzhou 730000,Gansu Province,China;Institute of Integrated Traditional Chinese Medicine and Western Medicine,School of Basic Medical Sciences,Lanzhou University,Lanzhou 730000,Gansu Province,China;Department of Oral and Maxillofacial Surgery,Hospital of Stomatology Lanzhou University,Lanzhou 730000,Gansu Province,China)

机构地区：[1]兰州大学口腔医学院,甘肃兰州730000 [2]兰州大学基础医学院中西医结合研究所,甘肃兰州730000 [3]兰州大学口腔医院口腔外科,甘肃兰州730000

出　　处：《中国临床药理学杂志》2022年第9期970-973,988,共5页The Chinese Journal of Clinical Pharmacology

基　　金：中央高校基本科研基金交叉创新课题资助项目(lzujbky-2020-cd03)。

摘　　要：目的 探讨黄芪多糖(APS)如何影响荷腹水瘤小鼠的氧化应激功能。方法 取生长良好的S180细胞配成瘤细胞悬液接种于小鼠腹腔内,建立腹水瘤模型。将荷瘤小鼠随机分为模型组(腹腔注射0.9%NaCl 100 mL)、对照组(腹腔注射100 mg·kg^(-1)环磷酰胺100 mL)和低、中、高剂量实验组(分别腹腔注射20,40和60 mg·mL^(-1)APS 100 mL),每组30只;另取18只正常小鼠作为空白组(腹腔注射0.9%NaCl 100 mL)。6组小鼠均每天给药1次,连续给药28 d。用比色法测定血清、肝和脑组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、一氧化氮(NO)、髓过氧化物酶(MPO)和总抗氧化能力(T-AOC)的含量。结果 干预28 d后,APS使血清SOD水平由模型组中的(64.78±3.92)U·mL^(-1)升高至高剂量实验组的(102.35±9.84)U·mL^(-1),其余CAT、GSH-Px、T-AOC水平同样明显升高,使MDA水平由模型组中的(120.35±13.98)μmol·L^(-1)降低至高剂量实验组的(72.65±6.91)μmol·L^(-1),其余NO和MPO水平同样降低,且随给药浓度呈现一定的量效关系;并在肝、脑组织中也表现出相类似的关系。结论 APS可促进并改善荷瘤小鼠体内抗氧化活性,进而影响氧化应激功能。Objective To explore the effect of Astragalus polysaccharide(APS)on oxidative stress function in ascites tumor-bearing mice. Methods The well-growing S180 cells were mixed with tumor cell suspension and inoculated into the abdominal cavity of mice to establish ascites tumor model. The tumor-bearing mice were randomly divided into model group(intraperitoneally injected 0.9% NaCl 100 mL), control group(intraperitoneally injected 100 mg·kg^(-1)cyclophosphamide 100 mL), experimental-L, M, H groups(injected intraperitoneally with 20, 40 and 60 mg·mL^(-1)APS 100 mL, respectively) with 30 mice in each group;another 18 normal mice were taken as the blank group(intraperitoneally injected isotonic saline 100 mL). Mice in 6 groups were given continuous administration once a day for 28 days. Colorimetric method was used to determine the effects of APS on superoxide dismutase(SOD), malondialdehyde(MDA), catalase(CAT) and glutathione peroxidase(GSH-Px), nitric oxide(NO), myeloperoxidase(MPO) and total antioxidant capacity(T-AOC) activity among serum, liver and brain tissues. Results After 28 days of intervention, the serum SOD level increased from( 64. 78 ± 3. 92) U·mL^(-1)in model group to (102. 35 ± 9. 84) U·mL^(-1)in experimental-H group,and the levels of CAT,GSH-Px and T-AOC also increased significantly. MDA level decreased from (120. 35 ± 13. 98) μmol·L^(-1)in model group to( 72. 65 ± 6. 91) μmol ·L^(-1)in experimental-H group,NO and MPO levels also reduced,there was a dose-effect relationship with drug concentration. It also showed a similar relationship in liver and brain tissues. Conclusion APS can improve the antioxidant activity in tumor-bearing mice and affect the oxidative stress function.

关 键 词：黄芪多糖 腹水瘤 抗氧化 氧化应激 

分 类 号：R28[医药卫生—中药学]