高效锑氧化菌的筛选鉴定及其对土壤中锑迁移转化的影响  

Isolation and identification of a high efficiency antimony oxidizing bacterium and its effect on the migration and transformation of antimony in soil

作　　者：李姗颖 张立鑫 李梅[1] LI Shanying;ZHANG Lixin;LI Mei(School of municipal and environmental engineering,Shandong Jianzhu University,Jinan 250101,China;State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China)

机构地区：[1]山东建筑大学市政与环境工程学院,济南250101 [2]中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京100085

出　　处：《环境工程学报》2022年第5期1602-1609,共8页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金面上项目(42077299)。

摘　　要：为了筛选出高抗性的锑(Antimony,Sb)氧化菌株,并阐释该细菌对土壤中Sb迁移转化的机制,从Sb污染土壤中富集培养并分离出Sb高抗性和高氧化效率的菌株,结合细菌形态、生理生化检测、16S rDNA基因序列分析等方法,确定了菌株的分类地位;通过该细菌与Sb污染土壤共孵育实验,研究了细菌对土壤中Sb迁移转化的影响。结果表明,筛选得到的菌株为假单胞菌属Pseμdomonas,命名为Pseμdomonas sp.ZLX16;菌株ZLX16能够耐受2 mol·m^(−3)的Sb(Ⅲ)以及10 mol·m^(−3)的Sb(Ⅴ),并且在3 d内可以将100 mmol·m^(−3)的Sb(Ⅲ)完全氧化。土壤孵育实验表明,该菌株能够显著减少Sb从固相土壤到液相中的释放量达63.5%,对土壤颗粒表面的Sb(Ⅲ)有氧化作用,并且能够促进Sb(Ⅴ)以强吸附态的形式固定在土壤中;该菌株对Sb的氧化速率较快,并且可以显著降低土壤中Sb的流动性,对修复Sb矿区污染土壤具有潜在的应用价值。本研究结果可为了解微生物影响土壤中污染物的迁移转化机制提供参考。The purpose of this study was to isolate a highly efficient antimonite oxidizing strain and explore the mechanism of this strain’s the migration and transformation of antimony in soil.The taxonomic status of strain was determined by the morphological,physiological and biochemical identification,and 16S rDNA sequence analysis.The strain was incubated with Sb-contaminated soil to explore its effect on Sb mobility.The result suggested that the strain belonged to genus Pseμdomonas,named Pseμdomonas sp.ZLX16.It could tolerate 2 mol·m^(−3) Sb(Ⅲ)and 10 mol·m^(−3) Sb(Ⅴ).The oxidation experiment results showed that strain oxidized almost 100 mmol·m^(−3) Sb(Ⅲ)to Sb(Ⅴ)for 3 d.Soil incubation experiment suggested that strain ZLX16 decreased the Sb release from solid to liquid phase by 63.5%.Strain oxidized adsorbed Sb(Ⅲ)on the surface of soil and promoted the fixation of Sb(Ⅴ)in soil in the form of strongly adsorbed Sb.Strain ZLX16 had a high oxidation rate of Sb and could significantly inhibit Sb release from soil to water.The results of this study can provide a reference for understanding the migration and transformation mechanism of microorganisms affecting pollutants in soil.

关 键 词：锑氧化菌 分离鉴定 迁移转化 锑污染土壤 

分 类 号：X53[环境科学与工程—环境工程]