解淀粉芽胞杆菌ZF57微粉剂的研制及对黄瓜棒孢叶斑病的防治效果  

Preparation of Bacillus amyloliquefaciens ZF57 micropowder and its control effect on cucumber coryneform leaf spot

作　　者：苑宝洁 李磊 李新宇 杨文 石延霞[2] 柴阿丽[2] 张红杰[1] 谢学文[2] 李宝聚[2] YUAN Baojie;LI Lei;LI Xinyu;YANG Wen;SHI Yanxia;CHAI Ali;ZHANG Hongjie;XIE Xuewen;LI Baoju(Hebei North University,Zhang Jiakou 075000,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Beijing Plant Protection Station,Beijing 100029,China)

机构地区：[1]河北北方学院,张家口075000 [2]中国农业科学院蔬菜花卉研究所,北京100081 [3]北京市植物保护站,北京100029

出　　处：《植物病理学报》2022年第2期235-246,共12页Acta Phytopathologica Sinica

基　　金：国家重点研发计划(2017YFD0701301);中国农业科学院科技创新工程(CAAS-ASTIP-IVFCAAS);农业部园艺作物生物学与种质创制重点实验室项目;宁夏瓜菜产业协同创新中心(2017DC55)。

摘　　要：采用单因素试验和响应面分析等方法优化解淀粉芽胞杆菌ZF57的发酵工艺。得到解淀粉芽胞杆菌ZF57发酵的最优条件为山梨醇10 g·L^(-1)、棉籽饼粉17 g·L^(-1)、NaH_(2)PO_(4)0.5 g·L^(-1)、Na;HPO;0.4 g·L^(-1),初始pH 7.0、装液量80 mL/250mL三角瓶、接种量2.1%、培养温度30℃、转速180 r·min^(-1)、发酵时间32 h,此时含菌量达到3.8×10^(8)CFU·mL^(-1),较优化前提高了80%。明确了解淀粉芽胞杆菌微粉剂的最佳配方为解淀粉芽胞杆菌母粉50%,白炭黑10%,分散剂木质素磺酸钠1%,表面活性剂十二烷基硫酸钠4%,保护剂羧甲基纤维素钠1%,硅藻土补足至100%。该制剂含菌量为3.26×10^(9)CFU·mL^(-1),平均粒径8.31μm,分散指数98.16%,浮游性指数85.37,含水率1.42%,坡度角68°,各项检测结果均符合标准。解淀粉芽胞杆菌ZF57微粉剂对黄瓜棒孢叶斑病的田间防治效果高达97.12%。Single factor test and response surface analysis were used to optimize the fermentation process of Bacillus amyloliquefaciens ZF57. Results showed that the optimal medium and culture condition for strain ZF57 were as follows: sorbitol 10 g·L^(-1), cottonseed cake powder 17 g·L^(-1), NaH_(2)PO_(4)0.5 g·L^(-1)、Na;HPO;0.4 g·L^(-1), pH 7.0, 80 mL medium in 250 mL flask, inoculum concentration 2.1%, 30 ℃, 180 r·min^(-1), 32 h. Under the optimized condition, the yield of strain ZF57 reached to 3.8 × 10^(8) CFU·mL^(-1), increased by 80% in comparison with the initial fermentation process. Screened a nutritional auxiliary and the optimum formulation of Bacillus amyloliquefaciens ZF57 micropowder. 50% of satrin ZF57 mother powder as active ingredient, 10% silica as second carrier, 1% sodium ligninsulfonate as dispersant, 4% sodium dodecyl sulfate as surfactant, 1% carboxymethylcellulose as protector, diatomite makes up to 100%. The B. amyloliquefaciens ZF57 micropowder has a particle size of 8.31 μm and a dispersion index of 98.16%. The planktonic index is 85.37, the water content is 1.42%, The slope angle is 68°. These results were in line with formulation standard. And the control effect of Bacillus amyloliquefaciens ZF57 powder on cucumber coryneform leaf spot was as high as 97.12%.

关 键 词：解淀粉芽胞杆菌 发酵条件优化 微粉剂 防治效果 

分 类 号：S432.44[农业科学—植物病理学]