洛阳核桃黑斑病病原菌鉴定  

Identification of the pathogen of walnut black spot in Luoyang

作　　者：梁家乐 李学强[1] 丁文轩 LIANG Jiale;LI Xueqiang;DING Wenxuan(College of Horticulture and Plant Protection,Henan University of Science and Technology,Fruit cultivation and physiology laboratory,Luoyang 471000,China)

机构地区：[1]河南科技大学园艺与植物保护学院,果树栽培生理实验室,洛阳471023

出　　处：《植物病理学报》2022年第2期286-290,共5页Acta Phytopathologica Sinica

基　　金：河南省科技公关资助项目(192102110038)。

摘　　要：0引言核桃黑斑病是核桃的三大主要病害之一,发病范围广,危害严重,一般植株受害率70%~90%,果实受害率10%~40%,严重时达65%以上,导致核桃栽培经济效益大幅降低[1]。核桃黑斑病病菌在病枝、芽鳞和残留的病果等处越冬,从气孔、皮孔、柱头、伤口等处侵入,借雨水、花粉、昆虫传播,可多次侵染[2]。In order to explore the pathogenic bacteria of walnut black spot in Luoyang, a field survey was carried out in Luoyang walnut orchard in 2020, typical disease samples were collected, and the pathogenic bacteria were isolated by tissue separation method. After purification and culture, the pathogens were connected to healthy leaves by acupuncture method, and the pathogenicity was tested and Koch′s rule was verified. The isolated strain YSYB was identified by morphological characteristics, physiological and biological identification, and the results showed that the strain YSYB was very similar to Pantoea. Using the total DNA of the strain as a template, the 16 S rDNA universal primer was used for PCR amplification. Agarose gel electrophoresis showed a fragment of about 1 500 bp in length, and sequencing results showed that the amplified 16 S rDNA fragment was 1 450 bp in length. The results showed that the 16 S rDNA sequence of strain YSYB was 100% similar to Pantoea agglomerans by comparison with the known sequence in GenBank, and the phylogenetic tree was clustered into the same branch. The results showed that the pathogen was P. agglomerans.

关 键 词：受害率 发病范围 核桃栽培 病原菌鉴定 核桃黑斑病 皮孔 芽鳞 危害严重 

分 类 号：S436.64[农业科学—农业昆虫与害虫防治]