新生大鼠原始卵泡凋亡与PI3K/Akt/Foxo3a信号通路的相关性  

Correlation between apoptosis of mordial follicles of the newborn rats and PI3K/Akt/Foxo3a signaling pathway

作　　者：赵粉琴[1] 丁晓南 安明霞 张小花[1] 贾学玲[2] ZHAO Fenqin;DING Xiaonan;AN Mingxia;ZHANG Xiaohihua;JIA Xueling(Gansu University of Traditional Chinese Medicine,Lanzhou,Gansu Province;Reproductive Center of The First Hospital of Lanzhou University,Lanzhou)

机构地区：[1]甘肃中医药大学,兰州730000 [2]兰州大学第一医院

出　　处：《中国计划生育学杂志》2022年第6期1227-1232,共6页Chinese Journal of Family Planning

基　　金：国家自然科学基金(81760806);2019年甘肃省中医药管理局项目(GZK-2019-28);2019年甘肃省高等学校创新能力提升项目(2019B-103);甘肃省教育厅项目(0706-05)。

摘　　要：目的:观察PI3K激动剂对新生大鼠卵巢PI3K/Akt/Foxo3a蛋白、细胞周期以及凋亡蛋白表达的影响。方法:取7日龄雌性大鼠卵巢体外培养,随机分为对照组、PI3K激活剂组、PI3K通路抑制剂组,每组10只。培养7dHE染色观察培养5d卵巢组织卵泡形态学;荧光显微镜法检测卵巢细胞凋亡;Western blot法比较p-AKT、p-FOXO3A、CDK2、P27、BCL-2及TAP63蛋白表达;PCR法检测培养3,5,7d PI3K、Akt、Foxo3a、Bcl-2、p27、Cdk2和Tap63 mRNA表达量。结果:PI3K激活剂组卵巢组织PI3K、p-AKT、p-FOXO3A、BCL-2、CDK2和TAP63蛋白表达增加(P<0.01),P27蛋白表达减少,尤以第5天最显著(P<0.05),之后逐渐下降,第7天达到最低(P<0.05);而PI3K抑制剂组卵巢组织PI3K、p-AKT、p-FOXO3A、CDK2、BCL-2和TAP63蛋白表达减少,P27蛋白表达增加。结论:激活PI3K/AKT/Foxo3a信号通路可以促进原始卵泡发育,其机制可能与上调卵巢组织PI3K、AKT、FOXO3A、BCL-2、CDK2和TAP63蛋白表达,下调P27蛋白表达有关。Objective:To observe the effects of PI3K agonist on the expression of PI3K/Akt/Foxo3a protein levels,cell cycle,and apoptosis protein of ovary of newborn rats.Methods:The ovaries of female rats of aged in 7 days(7 d)were cultured in vitro and were randomly divided into control group(the ovaries of the rats were cultured without PI3K activator or PI3K pathway inhibitor for 7 d),group A(the ovaries of the rats were cultured by PI3K activator for 7 d),and group B(the ovaries of the rats were cultured by PI3K pathway inhibitor for 7 d).The follicular morphology of ovarian tissue cultured for 5 d was observed by HE staining,and the apoptosis of ovarian cells was detected by fluorescence microscope method.Western blot was used to compare the protein expressions of P-Akt,P-Foxo3a,CDK2,P27,Bcl-2,and TAP63,and PCR was used to detect the mRNA expressions of PI3K,Akt,Foxo3a,Bcl-2,p27,Cdk2,and Tap63 in the ovarian tissues on the 3rd d,5th d,and 7th d after culture.Results:The protein expressions of PI3K,p-AKT,p-FOXO3 A,BCL-2,CDK2,and TAP63 in the ovarian tissues of the rats in group A had increased significantly(P<0.01),especially on the 5 th day after culture(P<0.05),but then which had decreased gradually after the 7 th day after culture(P<0.05).The protein expression of P27 protein of the rats in group A had decreased significantly.The protein expressions of PI3K,p-AKT,p-FOXO3 A,BCL-2,CDK2,and TAP63 in ovarian tissues of the rats in group B had increased significantly(P<0.01),but the protein expression of P27 protein of the rats had decreased significantly.Conclusion:Activation of PI3K/AKT/Foxo3a signaling pathway can promote the development of primitive follicles,and the mechanism of which may be related to the up-regulation of the protein expression of PI3K,AKT,FOXO3 A,BCL-2,CDK2,and TAP63 in ovarian tissues,and related to the down-regulation of the expression of P27 protein.

关 键 词：新生大鼠 原始卵泡凋亡 PI3K/Akt/Foxo3a信号通路 P27蛋白 BCL-2蛋白 

分 类 号：R339.22[医药卫生—人体生理学]