甘肃省玉米鞘腐病病原菌鉴定及生物学特性观察  

Dentification and Biological Characteristics of Pathogens of Sheath Rot of Maize in Gansu Province

作　　者：常浩 李文学 徐志鹏 周琦 汪亮芳 吴之涛 马金慧 杨克泽 张建超 任宝仓 CHANG Hao;LI Wen-xue;XU Zhi-peng;ZHOU Qi;WANG Liang-fang;WU Zhi-tao;MA Jin-hui;YANG Ke-ze;ZHANG Jian-chao;REN Bao-cang(Gansu Academy of Agri-engineering Technology/Gansu Green Prevention and Control Engineering Research Center of Maize Diseases and Insect Pests/Wuwei Green Maize Pest and Disease Control Technology Innovation Center,Wuwei 733006;Zhangye Plant Protection and Inspection Station,Zhangye 733006,China)

机构地区：[1]甘肃省农业工程技术研究院/甘肃省玉米病虫害绿色防控工程研究中心/武威市玉米病虫害绿色防控技术创新中心,甘肃武威733006 [2]张掖市植保植检站,甘肃张掖734000

出　　处：《玉米科学》2022年第2期168-175,共8页Journal of Maize Sciences

基　　金：甘肃省科技计划重点研发项目(18YF1NA011);甘肃省陇原青年人才创新项目(个人);定西市科技计划项目-玉米病虫害关键防控技术集成与应用(DX2021AR09)。

摘　　要：2021年6月在甘肃省张掖市甘州区党寨镇自然感玉米鞘腐病的玉米植株中分离鉴定病原菌并观察分析病原菌的生物学特性。结果表明,该致病菌株(QF-1RC)形态学结构与拟轮枝镰孢菌(Fusarium verticillioides)一致。基于rDNA-ITS和微管蛋白序列分析发现,该病原菌与拟轮枝镰孢菌MN652651和FR997663的相似度达99%,结合形态学和与分子生物学的鉴定结果将其定为拟轮枝镰孢菌。生物学特性观察发现,菌丝生长最适温度为25℃,弱酸弱碱环境下均有利于菌丝生长,全光照环境条件下菌丝生长速率快,不同C、N源对菌丝生长和产孢量有一定的影响,D-纤维二糖培养基上菌丝生长相对受限,在海藻糖和D-半乳糖培养基上菌株产孢量较多,硫酸铵培养基上病原菌生长相对受限,乙酸铵培养基上菌株产孢量最多,硝酸钠培养基上菌株产孢量较少。In June 2021,pathogens were isolated and identified from corn plants of naturally susceptible corn sheath rot in Dangzhai Town,Ganzhou District,Zhangye City,Gansu Province,and their biological characteristics were observed and analyzed.The results show that:The morphological structure of this pathogenic strain(QF-1RC)was consistent with that of F.verticillioides.Based on rDNA-ITS and tubulin sequence analysis,the similarity be⁃tween QF-1RC and F.Verticillioides(MN652651 and FR997663)reached 99%.Combined with morphological and molecular biological identification results,it was identified as F.verticillioides.It was found that the optimum tem⁃perature for mycelium growth was 25℃,The environment of weak acid and weak alkali was conducive to mycelium growth,The growth rate of mycelia was fast under full light,Different C and N sources had certain effects on myceli⁃al growth and spore production,The mycelial growth was relatively limited on D-cellose medium,while the spore production was more on trehalose and D-galactose medium.The growth of pathogenic bacteria was relatively limited on the(NH_(4))_(2)SO_(4) medium,the spore production of bacterial strains on the ammonium acetate medium was the most,while the spore production of bacterial strains on the NaNO_(3) medium was less.

关 键 词：玉米 鞘腐病 拟轮枝镰孢菌 生物学特性 甘肃 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]