七氟醚上调微小RNA-203对胶质瘤细胞侵袭和迁移的影响  

Effects of sevoflurane up-regulating miR-203 on the invasion and migration of glioma cells

作　　者：姚梦夏[1] 王淋[1] 周颖[1] 郑辉哲[1] YAO Meng-xia;WANG Lin;ZHOU Ying;ZHENG Hui-zhe(Department of Anesthesiology,Fujian Cancer Hospital,Fujian Medical University Cancer Hospital,Fuzhou 350014,Fujian Province,China)

机构地区：[1]福建省肿瘤医院福建医科大学附属肿瘤医院麻醉科,福建福州350014

出　　处：《中国临床药理学杂志》2022年第10期1055-1059,共5页The Chinese Journal of Clinical Pharmacology

基　　金：福建省科技计划基金资助项目(2018Y2003)。

摘　　要：目的研究七氟醚(Sevo)上调微小RNA-203(miR-203)对胶质瘤细胞侵袭、迁移的影响。方法将胶质瘤U87细胞分为对照组、空载组、七氟醚组、七氟醚+空载组、miR-203转染组、七氟醚+miR-203转染组。对照组未转染细胞;空载组给予细胞转染空质粒处置;七氟醚组将未转染的细胞直接给予4%Sevo处置;七氟醚+空载组给予在细胞转染空质粒后给予4%Sevo处置;miR-203转染组给予细胞转染重组质粒处置;七氟醚+miR-203转染组在细胞转染重组质粒后给予4%Sevo处置。用噻唑蓝(MTT)法测定细胞增殖情况,用实时荧光定量聚合酶链反应法检测miR-203的表达水平,用Transwell实验测定细胞侵袭和迁移能力。结果MTT实验筛选出Sevo作用浓度为4%,用于后续实验。空载组、对照组、miR-203转染组的miR-203相对表达水平分别为1.01±0.12,1.03±0.11和2.08±0.22,miR-203转染组的miR-203相对表达水平与空载组、对照组比较,差异均有统计学意义(均P<0.05)。对照组、七氟醚组、miR-203转染组、七氟醚+miR-203转染组的侵袭数分别为(99.82±5.67),(51.06±3.31),(68.24±4.28)和(34.18±1.57)个,迁移细胞数分别为(73.24±4.48),(48.64±3.15),(56.47±3.42)和(31.05±1.53)个,对照组、七氟醚+miR-203转染组的上述指标分别与七氟醚组、miR-203转染组比较,差异均有统计学意义(均P<0.05)。结论Sevo可能通过上调miR-203表达水平,从而抑制胶质瘤U87细胞侵袭、迁移。Objective To study the effects of sevoflurane(Sevo)up-regulating microRNA-203(miR-203)on the invasion and migration of glioma cells.Methods Glioma U87 cells were divided into control,empty,Sevo group,Sevo+empty,miR-203 transfection and Sevo+miR-203 transfection groups.Control group was untransfected cells.Empty group was treated with cells transfected with empty plasmid.In Sevo group,untransfected cells were directly treated with 4%Sevo.Sevo+empty group was given 4%Sevo treatment after cells were transfected with empty plasmid.The miR-203 transfection group was treated with recombinant plasmid transfected cells;the Sevo+miR-203 transfection group was treated with 4%Sevo after the cells were transfected with the recombinant plasmid.Methyl thiazolyl tetrazolium(MTT)assay was used to detect cell proliferation,quantitative real-time polymerase chain reaction was used to detect the expression of miR-203,and Transwell assay was used to detect cell invasion and migration.Results The concentration of Sevo was selected as 4%by MTT test for subsequent experiments.The relative expression levels of miR-203 in empty,control and miR-203 transfection groups were 1.01±0.12,1.03±0.11 and 2.08±0.22;the relative expression level of miR-203 in miR-203 transfection group was compared with those of empty and control groups,and the differences were statistically significant(all P<0.05).The invasion cells number of control,Sevo,miR-203 transfection,Sevo+miR-203 transfection groups was(99.82±5.67),(51.06±3.31),(68.24±4.28)and(34.18±1.57)and the migration cells number was(73.24±4.48),(48.64±3.15),(56.47±3.42)and(31.05±1.53).The above indicators of the control group and the sevoflurane+miR-203 transfection group were compared with those of sevoflurane group and miR-203 transfection group respectively,and the differences were statistically significant(all P<0.05).Conclusion Sevo may inhibit the invasion and migration of U87 cells by up-regulating the expression of miR-203.

关 键 词：七氟醚 微小RNA-203 胶质瘤细胞 侵袭 迁移 

分 类 号：R971.2[医药卫生—药品]