黄芪注射液对肺结核大鼠免疫功能、自噬和凋亡的影响  

Effect of Astmgali Radix injection on immune function,autophagy and apoptosis in pulmonary tuberculosis rats

作　　者：侯良绢[1] 李晓朋 赵春娥 陈永宏 刘刚 HOU Liang-juan;LI Xiao-peng;ZHAO Chun-e;CHEN Yong-hong;LIU Gang(Department of Anatomy,Chongqing Three Gorges Medical College,Chongqing 404120,China;Department of Infection,Three Gorges Hospital Affiliated to Chongqing University,Chongqing 404000,China)

机构地区：[1]重庆三峡医药高等专科学校解剖学教研室,重庆404120 [2]重庆大学附属三峡医院感染科,重庆404000

出　　处：《中国临床药理学杂志》2022年第10期1092-1096,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究黄芪注射液对肺结核大鼠免疫功能、自噬和凋亡的影响及机制。方法将SD大鼠分成对照组、模型组(注射结核杆菌)、实验组(注射结核杆菌,8 mL·kg^(-1)黄芪注射液处理)、实验+LY294002组[注射结核杆菌,8 mL·kg^(-1)黄芪注射液和磷脂酰肌醇-3激酶蛋白激酶B(PI3K/Akt)信号抑制药LY294002处理]。以蛋白质印迹(Western blot)法检测肺组织中磷脂酰肌醇-3激酶(PI3K)、蛋白激酶B(Akt)、磷酸化Akt(p-Akt)、Beclin1蛋白表达,用酶联免疫吸附(ELISA)法检测血清中炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)水平,以流式细胞术检测T细胞亚群,以脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测细胞凋亡。结果对照组、模型组、实验组、实验+LY294002组PI3K蛋白表达水平为0.59±0.05,0.23±0.03,0.46±0.05和0.33±0.02,p-Akt/Akt表达水平为0.40±0.04,0.22±0.02,0.42±0.06和0.29±0.04,TNF-α表达量为(13.09±1.47),(81.93±5.85),(39.98±4.83)和(65.98±3.91)pg·mL^(-1),IL-1β表达量为(15.36±1.65),(90.46±6.70),(21.31±2.68)和(45.22±6.24)pg·mL^(-1),IL-6表达量为(30.28±3.01),(193.14±20.91),(54.56±6.94)和(86.30±7.90)pg·mL^(-1),T细胞亚群CD^(3+)水平为(48.51±3.89)%,(34.26±2.98)%,(46.87±5.97)%和(37.37±4.58)%,CD^(4+)水平为(39.29±3.92)%,(22.99±2.68)%,(37.46±1.87)%和(30.27±2.94)%,细胞凋亡指数(5.27±0.76)%,(33.72±2.50)%,(16.58±1.58)%和(27.39±2.16)%,Beclin1蛋白表达水平为0.22±0.03,0.56±0.07和0.25±0.02,0.45±0.06。以上指标,模型组与对照组比,差异均有统计学意义(均P<0.05);实验组与模型组比,差异均有统计学意义(均P<0.05)。结论黄芪注射液通过激活PI3K/Akt信号改善肺结核大鼠免疫功能,减轻炎症,抑制细胞自噬、凋亡。Objective To study the effect and mechanism of Astmgali Radix injection on immune function,autophagy and apoptosis in pulmonary tuberculosis rats.Methods Rats were divided into control group,model group(injection of mycobacterium tuberculosis),experimental group(injection of mycobacterium tuberculosis,8 mL·kg^(-1) astmgali radix injection treatment),experimental+LY294002 group[injection of mycobacterium tuberculosis,8 mL·kg^(-1) astmgali radix injection and phospoinositide 3-kinase/protein kinase B(PI3K/Akt)signal inhibitor LY294002treatment].Western blot method was used to detect phosphatidylinositol-3 kinase(PI3K),protein kinase B(Akt),p-Akt,Beclin1 protein expression,enzyme linked immunosorbent assay(ELISA)method was used to detect serum inflammatory factor tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6)levels,flow cytometry was used to detect T cell subsets,deoxynucleotide terminal transferase-mediated nick end labeling(TUNEL)method was used to detect cell apoptosis.Results The expression levels of PI3K protein in control group,model group,experimental group,and experimental+LY294002 group were 0.59±0.05,0.23±0.03,0.46±0.05,0.33±0.02;the expression levels of p-Akt/Akt were 0.40±0.04,0.22±0.02,0.42±0.06,0.29±0.04;TNF-αexpression were(13.09±1.47),(81.93±5.85),(39.98±4.83),(65.98±3.91)pg·mL^(-1);IL-1βexpression were(15.36±1.65),(90.46±6.70),(21.31±2.68),(45.22±6.24)pg·mL^(-1),IL-6 expression were(30.28±3.01),(193.14±20.91),(54.56±6.94),(86.30±7.90)pg·mL^(-1);the CD3+levels of T cell subsets were(48.51±3.89)%,(34.26±2.98)%,(46.87±5.97)%,(37.37±4.58)%;the CD4+levels of T cell subsets were(39.29±3.92)%,(22.99±2.68)%,(37.46±1.87)%,(30.27±2.94)%;apoptosis rates were(5.27±0.76)%,(33.72±2.50)%,(16.58±1.58)%,(27.39±2.16)%,Beclin1 protein expression levels were 0.22±0.03,0.56±0.07,0.25±0.02,0.45±0.06.Compared model group with control group,the difference were statistically significant(all P<0.05);compared experimental group with model group,the diffe

关 键 词：黄芪 肺结核 自噬 凋亡 免疫功能 磷脂酰肌醇-3激酶(PI3K)/蛋白激酶B(Akt)信号 

分 类 号：R28[医药卫生—中药学]