三七皂苷Fc对SD大鼠心室肌细胞钠离子通道电流的影响  

Effects of Notoginsenoside Fc on Sodium Ion Channel Currents in Ventricular Myocytes of SD Rats

作　　者：钟飞 李梦婷 杜娅娅 许正新[1,2,3,4] ZHONG Fei;LI Mengting;DU Yaya;XU Zhengxin(Department of Pharmacology,School of Medicine,Yangzhou University,Yangzhou 225000,China;Key Joint Center of Infectious Diseases and Zoonosis Prevention and Control of Jiangsu Province,Yangzhou 225001,China;Key Laboratory of Integrative Medicine in Geriatrics Control of Jiangsu Province,Yangzhou 225001,China;Key Laboratory of Experimental&Translational Non-coding RNA Research of Jiangsu Province,Yangzhou 225009,China)

机构地区：[1]扬州大学医学院药学系,江苏扬州225000 [2]江苏省重点动物传染病和人畜共患病预防控制共创中心,江苏扬州225001 [3]江苏省中西医结合老年病防治重点实验室,江苏扬州225001 [4]江苏省非编码RNA基础与临床转化重点实验室,江苏扬州225009

出　　处：《中国现代应用药学》2022年第9期1162-1167,共6页Chinese Journal of Modern Applied Pharmacy

基　　金：扬州大学大学生科研创新计划项目(X20200782)。

摘　　要：目的探究三七皂苷Fc(notoginsenoside Fc,N-Fc)对SD大鼠心室肌细胞钠离子通道电流的影响。方法使用Langendorff恒温恒压灌流装置通过酶解法急性分离SD大鼠心室肌细胞,采用标准全细胞膜片钳技术观察并记录不同浓度的N-Fc对大鼠心室肌细胞钠通道电流(I_(Na))及其动力学特征的影响。结果5μmol·L^(–1)的N-Fc对I_(Na)无明显影响,随着药物浓度的增加,10,20,50μmol·L^(–1)的N-Fc可使钠峰值电流由给药前的(–87.49±3.40)pA/pF依次下降为(–62.91±2.37),(–49.30±1.27),(–34.68±3.21)pA/pF(P<0.01);I_(Na)的I-V曲线上移,但形态轨迹、激活电位及峰电位基本不变;I_(Na)的激活曲线向去极化方向迁移,半数激活电压由给药前的(–52.37±1.32)mV变为(–42.74±0.37),(–38.92±2.77),(–33.78±0.96)mV(P<0.05);失活曲线显著左移,半数失活电压(V_(1/2-in))由(–55.95±4.88)mV依次变为(–64.29±1.77),(–69.57±3.47),(–73.32±3.79)m V(P<0.05);此外,N-Fc能够显著延长I_(Na)失活后恢复时间,时间常数τ值(给药前,10,20,50μmol·L^(–1)N-Fc处理后)分别为(12.50±1.17),(21.36±2.23),(25.35±1.25),(34.35±1.24)ms(P<0.05)。结论N-Fc能浓度依赖性地抑制SD大鼠心室肌细胞的I_(Na),并能显著影响其激活、失活及失活后恢复的动力学特征。OBJECTIVE To explore the effects of notoginsenoside Fc(N-Fc) on sodium ion channel currents of ventricular myocytes in SD rats.METHODS Ventricular myocardial cells were enzymatically isolated from SD rats by Langendorff with constant pressure and temperature,the whole-cell patch clamp techniques were used to observe and record the effects of I_(Na) and its kinetics in ventricular mgocytes of SD rats treated with different concentrations of N-Fc.RESULTS There was no significant effect on I_(Na) when the concentration of N-Fc was 5 μmol·L^(–1),with the increase of drug concentration,the peak sodium current was decreased from (–87.49±3.40)pA/pF to (–62.91±2.37),(–49.30±1.27),(–34.68±3.21)pA/pF(P<0.01) under the effects of 10,20 and 50 μmol·L^(–1) N-Fc.I-V curve of I_(Na) were shifted upward,but the morphological trajectories,activation potential and peak potential did not change.The activation curve of I_(Na) were shifted to the depolarization direction,and the half in activation voltage was changed from (–52.37±1.32)mV to (–42.74±0.37),(–38.92±2.77),(–33.78±0.96)mV(P<0.05).The inactivation curve shifted significantly to the left,the half inactivation voltage changed from (–55.95±4.88)mV to (–64.29±1.77),(–69.57±3.47),(–73.32±3.79)mV(P<0.05).In addition,N-Fc significantly prolonged the recovery time after I_(Na) inactivation,and the time constant τ(before administration,after treated with 10,20,50 μmol·L^(-1 )N-Fc) were (12.50±1.17),(21.36±2.23),(25.35±1.25),(34.35±1.24)ms(P<0.05).CONCLUSION N-Fc can inhibit I_(Na) of SD rats ventricular myocytes in a concentration-dependent manner and it has an significant effect on the kinetics characteristics of activation,inactivation and recovery.

关 键 词：三七皂苷Fc 膜片钳 钠通道 心室肌细胞 SD大鼠 

分 类 号：R965.2[医药卫生—药理学]