运动应激对衰老大鼠学习记忆能力及海马线粒体UCP4、Caveolin-1蛋白的影响  

Effects of exercise stress on learning and memory ability and UCP4 and caveolin-1 protein in hippocampal mitochondria of aging rats

作　　者：刘忠民 吕振磊 尹超 LIU Zhong-min;LV Zhen-lei;YIN Chao(College of Health Sciences,Hainan Vocational University of Science and Technology,Haikou 571126,China)

机构地区：[1]海南科技职业大学健康科学学院,海南海口571126

出　　处：《中国实验诊断学》2022年第4期590-595,共6页Chinese Journal of Laboratory Diagnosis

基　　金：海南省高等学校科学研究项目(HnKY2019ZD-41);海南省自然科学基金资助(821QN272)。

摘　　要：目的 探讨运动应激对神经系统的保护作用机制。方法 6周龄SPF级雄性SD大鼠40只,随机分为4组:对照组(C)、D-半乳糖注射组(D)、实验组(DE)、运动组(E);连续8周腹腔注射D-半乳糖建立衰老模型;8周中等强度的跑台运动干预建立运动应激模型;用微量法测定线粒体复合物Ⅲ的活性,免疫组化及Western Blot方法评估运动应激对海马线粒体UCP4、Caveolin-1蛋白表达的影响。结果 (1)Morris水迷宫结果显示,DE、E组大鼠路径、潜伏期、平均穿越次数均优于D组;(2)D组大鼠海马中UCP4蛋白表达最低,与C组及DE组相比均降低(P<0.05-0.01);E与DE组相比UCP4蛋白表达上调(P<0.05);(3)Caveolin-1在D组中表达最多,与C相比显著上调(P<0.01);DE组与C组相比Caveolin-1蛋白表达上调(P<0.05)。(4)与C组相比,DE、E组线粒体复合物Ⅲ的酶活性均增加(P<0.05-0.01)。结论 运动应激可以调控大鼠海马线粒体Caveolin-1蛋白的表达,上调海马线粒体UCP4蛋白,增加线粒体呼吸链酶活性,延缓并改善在衰老过程中的大脑认知机能退行性或轻度认知功能障碍。Objective To explore the protective mechanism of exercise stress on nervous system.Methods Forty 6-week-old SPF male SD rats were randomly divided into four groups:control group(C),D-galactose injection group(D),experimental group(DE)and exercise group(E);The aging model was established by intraperitoneal injection of D-galactose for 8 weeks;The exercise stress model was established after 8 weeks of moderate intensity treadmill exercise intervention;The activity of mitochondrial complex III was measured by micro method,and the effects of exercise stress on the expression of UCP4 and caveolin-1 protein in hippocampal mitochondria were evaluated by immunohistochemistry and Western blot.Results(1)Morris water maze showed that the route,latency and average crossing times of rats in de and e groups were better than those in D group;(2)The expression of UCP4 protein in hippocampus of group D was the lowest,which was lower than that of group C and DE(P<0.05-0.01);Compared with DE group,the expression of UCP4 protein was up-regulated in E group(P<0.05);(3)Caveolin-1 was most expressed in group D and significantly up-regulated compared with group C(P<0.01);The expression of caveolin-1 protein was up-regulated in DE group compared with C(P< 0.05).(4)Compared with group C,the enzyme activity of mitochondrial complex III in DE and C groups increased(P<0.05-0.01).Conclusion Exercise stress can regulate the expression of caveolin-1 protein in rat hippocampal mitochondria,up-regulate UCP4 protein in hippocampal mitochondria,increase mitochondrial respiratory chain enzyme activity,delay and improve brain cognitive function degeneration or mild cognitive impairment in the process of aging.

关 键 词：运动应激 海马线粒体 UCP4 CAVEOLIN-1 

分 类 号：R338.64[医药卫生—人体生理学]