白藜芦醇调控hsa-miR-4689-3p、hsa-miR-4741-3p保护肾小球系膜细胞  

Resveratrol targets hsa-mir-4689-3p and hsa-miR-4741-3p to protect glomerular mesangial cells

作　　者：张铭 于昕新 浦荭秋 田阳 韩铠禧 赵天月 梁竞天 杨果 闵钰淇 石艳[1] ZHANG Ming;YU Xin-xin;PU Hong-qiu(Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学药学院,吉林长春130021

出　　处：《中国实验诊断学》2022年第5期755-758,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技厅国际合作项目(20190701045GH)。

摘　　要：目的 通过研究白藜芦醇对高糖下肾小球系膜细胞hsa-miR-4689-3p、hsa-miR-4741-3p表达的影响,探讨白藜芦醇对高糖下肾小球系膜细胞的保护作用及机制。方法将肾小球系膜细胞分为空白对照组(Con),高糖组(HG)和白藜芦醇组(Res),real-time PCR技术检测hsa-miR-4689-3p、hsa-miR-4741-3p在各组的相对表达量,采用生物信息学软件预测靶基因。结果 Real-time PCR结果表明,hsa-miR-4689-3p与hsa-miR-4741-3p的相对表达量在HG组中较Con组明显降低。与HG组相比,Res组肾小球系膜细胞中的相对表达量明显升高。靶基因预测发现hsa-miR-4689有OPA3,HCN4,HPSE等75个靶基因,其中与DN密切相关的靶基因有FASN、PAX2、PTPN5、ANGPT2等,hsa-miR-4741有CILP2,MMAB,POR等53个靶基因,其中与DN密切相关的靶基因有CD4、PLVAP、GAS2L1、SMURF1、KDM4B、SERPINA3、CACNA1C、DDR1等。结论 白藜芦醇对高糖诱导肾小球系膜细胞的保护作用可能部分通过调节hsa-miR-4689-3p与hsa-miR-4741-3p实现。Objective To study the effect of resveratrol on the expression of hsa-mir-4689-3p and hsa-miR-4741-3p in glomerular mesangial cells under high glucose,and to explore the protective effect and mechanism of resveratrol on glomerular mesangial cells under high glucose.Methods glomerular mesangial cells were divided into HG group,Res group,Con group.Real time PCR was used to detect the relative expression of hsa-mir-4689-3p and hsa-miR-4741-3p in each group,and bioinformatics software was used to predict the target genes.Results Real-time PCR verification results showed that the relative expression levels of hsa-miR-4689-3p and hsa-miR-4741-3p were significantly lower in the HG group than in the Con group.Compared with the HG group,the relative expression level of glomerular mesangial cells in the Res group was significantly increased.According to the prediction of target genes,hsa-mir-4689has 75target genes such as OPA3,HCN4and HPSE,among which FASN,PAX2,PTPN5and ANGPT2are closely related to DN;hsa-mir-4741has 53target genes such as CILP2,MMAB and POR,among which CD4,PLVAP,GAS2L1,SMURF1,KDM4B,SERPINA3,CACNA1Cand DDR1are closely related to DN.Conclusion The protective effect of resveratrol on high glucose induced glomerular mesangial cells may be partially realized by regulating hsa-miR-4689-3p and hsa-miR-4741-3p.

关 键 词：白藜芦醇 MICRORNA 肾小球系膜细胞 real-time PCR 

分 类 号：R965.2[医药卫生—药理学] R966[医药卫生—药学]