深海冷泉草酸青霉Penicillium oxalicum 13-37的化学成分研究  

Chemical constituents of Penicillium oxalicum 13-37 from cold-seep sediments

作　　者：刘雅萍 方圣涛[1] 时振振 季乃云[1] LIU Ya-ping;FANG Sheng-tao;SHI Zhen-zhen;JI Nai-yun(Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院烟台海岸带研究所,山东烟台264003 [2]中国科学院大学,北京100049

出　　处：《海洋科学》2022年第5期115-121,共7页Marine Sciences

基　　金：国家重点研发计划项目(2018YFC0310800);泰山学者青年项目(tsqn201909164);国家重大科技基础设施“科学”号海洋科学综合考察船(KEXUE2018G28)。

摘　　要：为了丰富深海冷泉天然产物结构多样性,本文对深海冷泉沉积物来源的草酸青霉Penicillium oxalicum13-37的次生代谢产物进行了研究,通过硅胶柱层析、反相硅胶柱层析、Sephadex LH-20凝胶柱层析、制备薄层层析、半制备高效液相色谱方法,从其发酵产物中分离得到11个单体化合物,分别为seco-blennolide B(1)、blennolide B(2)、citreorosein(3)、emodine(4)、isorhodoptilometrin(5)、aloe-emodin(6)、2-acetylemodin(7)、11-O-acetylaloeemodin(8)、phaseolorin I(9)、penitholabene(10)和paecilomycine A(11)。其中,化合物1为新化合物,化合物10和11为首次从深海冷泉草酸青霉中分离得到的萜类化合物,并首次对化合物10的单晶数据进行报道。此外,活性测试结果表明,化合物1对东海原甲藻和海洋卡盾藻、副溶血弧菌具有一定的抑制作用,对卤虫表现出微弱的毒性。To enrich the structural diversity of natural products from a deep-sea cold seep,the secondary metabolites of Penicillium oxalicum 13-37,a fungus derived from cold-seep sediments,were investigated.Eleven compounds were obtained using silica gel column chromatography,reversed-phase silica gel column chromatography,Sephadex LH-20 gel column chromatography,preparative thin-layer chromatography,and semi-preparative high-performance liquid chromatography.They were identified as seco-blennolide B(1),blennolide B(2),citreorosein(3),emodine(4),isorhodoptilometrin(5),aloe-emodin(6),2-acetylemodin(7),11-O-acetylaloeemodin(8),phaseolorin I(9),penitholabene(10),and paecilomycine A(11).Among them,1 is a novel compound.Compounds 10 and 11 are known terpenoids isolated from cold seep-derived Penicillium oxalicum for the first time,and the single-crystal data of compound 10 are reported for the first time.Additionally,the activities of the new compound were tested.The results revealed that the new compound has inhibitory effects on the growth of Prorocentrum donghaiense,Chattonella marina,and Vibrio parahaemolyticus.Furthermore,compound 1 weakly inhibited Artemia salina.

关 键 词：深海冷泉 草酸青霉 次生代谢产物 化学成分 生物活性 

分 类 号：O629[理学—有机化学]