苦参对特应性皮炎模型小鼠皮损组织PAR-2、TrK-A蛋白表达的影响  

Effects of Kushen(Radix Sophorae Flavescentis)on the Expression of PAR-2 and TrK-A Protein in Skin Lesions of Atopic Dermatitis Model Mice

作　　者：宋雪[1] 王成祥[1] 袁姣姣 张新荣 王国蜜 高珊珊[1] 孙占学[1] SONG Xue;WANG Chengxiang;YUAN Jiaojiao;ZHANG Xinrong;WANG Guomi;GAO Shanshan;SUN Zhanxue(Third Affiliated Hospital,Beijing University of Chinese Medicine,Beijing,100029;Dongzhimen Hospital,Beijing University of Chinese Medicine;Dong Xiao Kou Community Health Service Center;Beijing University of Chinese Medicine)

机构地区：[1]北京中医药大学第三附属医学院,北京市100029 [2]北京中医药大学东直门医院 [3]北京市昌平区东小口社区卫生服务中心 [4]北京中医药大学

出　　处：《中医杂志》2022年第9期869-874,共6页Journal of Traditional Chinese Medicine

基　　金：国家留学基金青年骨干教师项目(201906555021);北京中医药大学2018年度青年教师项目(2018-JYBZZ-JS181)。

摘　　要：目的探讨苦参治疗特应性皮炎的可能作用机制。方法60只BALB/c雄性小鼠随机分为正常组、模型组、西替利嗪组和苦参高、中、低剂量组,每组10只。除正常组外,其余各组小鼠用卵清蛋白(OVA)诱导特应性皮炎模型,造模成功后,苦参高、中、低剂量组小鼠分别给予苦参3.75、2.50、1.25 g/(kg·d)灌胃,西替利嗪组给予西替利嗪片10 mg/(kg·d)灌胃,正常组和模型组给予10 ml/(kg·d)生理盐水灌胃。各组均每天灌胃2次,连续3周。分别于干预1、2、3周时观察小鼠每30min内搔抓次数;干预3周后采用HE染色观察皮损组织病理形态学变化,测定血清中P物质(SP)、降钙素基因相关肽(CGRP)、前列腺素E2(PGE2)、白三烯B4(LTB4)浓度,分别采用免疫印记和免疫组化法检测皮损组织中蛋白酶激活受体2(PAR-2)和酪氨酸激酶A(TrK-A)蛋白表达。结果与正常组同时间相比,模型组小鼠干预1、2、3周时搔抓次数显著增加(P<0.01);HE染色显示小鼠的表皮过度角化、真皮层增厚、炎细胞浸润;血清中PGE2、CGRP、LTB4和SP浓度明显升高;皮损组织中PAR-2和TrK-A蛋白表达增多(P<0.05或P<0.01)。与模型组同时间比较,除苦参低剂量组干预1周时外,西替利嗪组和苦参各剂量组各时间点小鼠搔抓次数显著降低(P<0.01);西替利嗪组和苦参各剂量组小鼠的表皮和真皮比模型组明显变薄,炎症细胞浸润明显减轻,且以西替利嗪组和苦参高剂量组改善明显;西替利嗪组和苦参各剂量组小鼠血清中PGE2、CGRP、LTB4和SP浓度均降低,皮损组织中PAR-2和TrK-A蛋白表达降低(P<0.05或P<0.01)。与苦参高剂量组同时间比较,苦参低剂量组小鼠搔抓次数明显升高(P<0.05)。西替利嗪组及苦参各剂量组组间两两比较,小鼠血清中PGE2、CGRP、LTB4和SP浓度,皮损组织中PAR-2和TrK-A蛋白表达差异均无统计学意义(P>0.05)。结论苦参可以改善特应性皮炎瘙痒程度,其机制可能与抑制皮损组�Objective To explore the possible mechanism of Kushen(Radix Sophorae Flavescentis)in the treatment of atopic dermatitis(AD).Methods Sixty BALB/c male mice were randomly divided into normal group,model group,cetirizine group and Kushen high-,medium-and low-dose groups,with 10 mice in each group.Except for the normal group,mice were induced with ovalbumin(OVA)to develop the atopic dermatitis model.After successful modeling,the mice in the Kushen high-,medium-and low-dose groups were given 3.75,2.50,and 1.25 g/(kg·d)of Kushen by gavage respectively;mice in the cetirizine group were given 10 mg/(kg·d)of cetirizine tablets,while those in the normal group and model group received 10 ml/(kg·d)of normal saline by gavage.All the interventions were administered twice a day for three consecutive weeks.The number of scratches over 30 minutes was observed after 1-,2-and 3-week treatment.After 3-week treatment,HE staining was used to assess the pathological changes of skin lesions,and the concentrations of substance P(SP),calcitonin gene-related peptide(CGRP),prostaglandin E2(PGE2)and leukotriene B4(LTB4)in serum were determined;immunoblotting and immunohistochemistry were used to detect the protein expressions of protease-activated receptor 2(PAR-2)and tyrosine kinase A(TrK-A)in skin lesions,respectively.Results Compared to the results measured after 1-,2-and 3-week treatment in the normal group,the number of scratches significantly increased at the corresponding time in the model group(P<0.01).Moreover,HE staining results showed that the epidermis in the model group was hyperkeratinized;the dermis was thickened,and inflammatory cells were infiltrated in the mice;the concentrations of PGE2,CGRP,LTB4 and SP significantly increased,and PAR2 and TrK-A protein expressions in skin lesions significantly increased(P<0.05 or P<0.01).Compared to the results measured after 1-,2,and 3-week treatment in the model group,the number of scratches significantly decreased at the corresponding time in the cetirizine group and three Kushen gr

关 键 词：特异性皮炎 瘙痒 苦参 蛋白酶激活受体-2 酪氨酸激酶A 

分 类 号：R285.5[医药卫生—中药学]