四盐酸精胺抑制血小板活化的初步研究  

Preliminary Study on Spermine Tetrahydrochloride Inhibition of Platelet Activation

作　　者：王静 胡艳 朱曼 胡芮维 张洪为 黄远帅 WANG Jing;HU Yan;ZHU Man(Department of Blood Transfusion,The Affiliated Hospital of Southwest Medical University 646000)

机构地区：[1]西南医科大学附属医院输血科,646000 [2]西南医科大学

出　　处：《临床输血与检验》2022年第3期315-319,共5页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：西南医科大学大学生创新创业项目(No.2021601、No.2021566)资助。

摘　　要：目的初步探究不同浓度的多胺类一氧化氮供体四盐酸精胺对血小板保存期内不同存储时间节点的血小板活化的影响,讨论相关作用机制。方法将储存1 d、3 d、5 d的单采血小板分别分为实验组(A、B、C、D、E组)和对照组(F组),向实验组分别加入10 mmol/L、20 mmol/L、30 mmol/L、40 mmol/L、50 mmol/L的四盐酸精胺40μL,向对照组中加入等量的PBS溶液。采用荧光酶标法检测血小板的一氧化氮含量,采用流式细胞仪检测血小板膜表面CD62p的表达。结果不同储存时间节点的单采血小板添加四盐酸精胺后,单采血小板的一氧化氮含量均有升高,CD62p表达减少,差异有统计学意义(P<0.05);添加的四盐酸精胺浓度越高,血小板一氧化氮浓度越高,CD62p表达越少,四盐酸精胺浓度为40 mmol/L左右后趋于稳定。结论多胺类一氧化氮供体四盐酸精胺可以向血小板提供一氧化氮,在一定程度上抑制血小板的活化,这与四盐酸精胺浓度有一定关系。Objective To preliminarily explore the effects of different concentrations of polyamine nitric oxide donor Spermine Tetrahydrochloride on platelet activation at different time points during platelet storage and to discuss its relevant mechanism.Methods The apheresis platelets stored for 1 d,3 d,and 5 d were divided into experimental groups(groups A,B,C,D,and E)and control groups(group F).40μL of 10 mmol/L,20 mmol/L,30 mmol/L,40 mmol/L,and 50 mmol/L Spermine Tetrahydrochloride were added to the experimental groups.And an equal amount of PBS solution were added to the control group.Fluorescence enzyme labeling method was used to detect the nitric oxide content of platelets,and flow cytometry was used to detect the expression of CD62p on the platelet membrane surface.Results After adding Spermine Tetrahydrochloride to apheresis platelets at different storage time points,the nitric oxide content of apheresis platelets increased,and the expression of CD62p decreased,which was statistically significant(P<0.05);The higher concentration of Spermine Tetrahydrochloride were added,the higher nitric oxide concentration and the lower the CD62p expression in the platelets,and when the concentration of Spermine Tetrahydrochloride reach to 40 mmol/L it tends to be stable.Conclusion Spermine Tetrahydrochloride,a polyamine nitric oxide donor,can provide nitric oxide to platelets and inhibit platelet activation to a certain extent,which is related to the concentration of Spermine Tetrahydrochloride.

关 键 词：血小板活化 血小板保存 一氧化氮供体 CD62P 

分 类 号：R457[医药卫生—治疗学]