2017~2020年度全国HLA低分辨基因分型检测能力验证结果分析  

National Proficiency Testing for HLA Low-Resolution Molecular Typing:2017-2020 Procedure

作　　者：张立群[1] 肖尧[1] 周晓阳[1] 许晓东[2] 蔡剑平[1] ZHANG Li-qun;XIAO Yao;ZHOU Xiao-yang(The Key Laboratory of Geriatrics,Beijing Institute of Geriatrics,Institute of Geriatric Medicine,Chinese Academy of Medical Sciences,Beijing Hospital/National Center of Gerontology of National Health Commission,P.R.China 100730)

机构地区：[1]北京医院,国家老年医学中心,国家卫生健康委北京老年医学研究所,国家卫生健康委老年医学重点实验室,中国医学科学院老年医学研究院,100730 [2]北京医院血液科,国家老年医学中心,中国医学科学院老年医学研究院,100730

出　　处：《临床输血与检验》2022年第3期353-357,共5页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的分析2017年~2020年度全国主要医院及第三方检测机构HLA低分辨基因分型检测(HLA-A/B/DRB1)的能力验证(PT)结果,评估实验室检测能力,为提升实验室HLA分型检测水平提供数据支持。方法对国家卫生健康委临床检验中心PT数据库中HLA分型评价数据进行统计分析,从检测方法、检测试剂、人员因素等多角度分析各实验室结果的产生错误的可能原因。结果2017年~2020年回报等位基因数据共计12096例,出现错误等位基因共计127例,错误类型主要分为分型技术错误(13例)和人为错误(114例);PT活动参与实验室从78家增加至96家,总体错误率为1.05%(127/12096),近四年实验室出现错误的比例分别为2.56%(2/78)、4.88%(4/82)、8.75%(7/80)、3.13%(3/96)。结论近4年我国HLA低分辨基因分型检测实验室仍有一定比例的错误率,错误类型以人为错误为主,需引起实验室检测人员尤其是出具报告人员的高度重视。Objective To improve HLA genotyping performance in clinical laboratory,the results of National Proficiency Testing(PT)for HLA-A/B/DRB1 low-resolution genotyping were collected and summarized from 2017 to 2020.Methods HLA genotyping results from 2017~2020 PT were analyzed and different kinds of errors were described and classified,and the possible reasons were also analyzed.Results The number of participant laboratories was increasing from 78 to 96 during 2017 and 2020.12096 HLA alleles were returned from the participants during the 4 years and total of 127 errors(1.05%)were found.These 127 errors were classified into two major types including 13 technical errors and 114 artificial errors.The proportion of laboratory which made mistakes was 2.56%(2/78)、4.88%(4/82)、8.75%(7/80)and 3.13%(3/96)in 2017,2018,2019 and 2020,respectively.Conclusion There were above 2.5%of laboratories with errors in HLA genotyping PT surveys past four years.More attentions should be greatly paid to clinical HLA genotyping test and the procedure of reporting.

关 键 词：人类白细胞抗原 低分辨基因分型 能力验证 

分 类 号：R446[医药卫生—诊断学]