Ag掺杂ZnO酪氨酸酶生物传感器检测邻苯二酚  

Tyrosinase biosensor based on Ag-doped ZnO to detect catechol

作　　者：张美奇 李纯 孙丽霞[1] 孙建华[1] 周利琴[1] 张友全[1] 裴锐南[1] 廖丹葵[1] ZHANG Meiqi;LI Chun;SUN Lixia;SUN Jianhua;ZHOU Liqin;ZHANG Youquan;PEI Ruinan;LIAO Dankui(Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology,School of Chemistry and Chemical Engineering,Guangxi University,Nanning,530004,China)

机构地区：[1]广西石化资源加工及过程强化技术重点实验室广西大学化学化工学院,南宁530004

出　　处：《环境化学》2022年第5期1559-1567,共9页Environmental Chemistry

基　　金：国家自然科学基金(51372043);广西自然科学基金(2017GXNSFDA198052);广西石油资源加工及过程加强化技术重点实验室主任基金(2020Z003)资助。

摘　　要：邻苯二酚对环境和人体具有危害作用,本实验用静电纺丝法制备了银掺杂氧化锌复合材料(Ag-ZnO),通过负载酪氨酸酶(Tyr)制备酪氨酸酶/银掺杂氧化锌/壳聚糖/玻碳生物电极(Tyr/Ag-ZnO/CS/GCE).利用扫描电子显微镜、X射线能谱分析、X射线粉末衍射和N2吸附脱附法对材料形貌、元素组成及比表面积进行表征.利用循环伏安法(CV)和交流阻抗法(EIS)对Tyr/Ag-ZnO/CS/GCE电极的电化学性能进行测试,利用计时电流法(IT)研究该电极对邻苯二酚(CA)的检测能力.结果表明,加入少量Ag可明显减小ZnO的颗粒尺寸,提高了复合材料导电性,Tyr/Ag-ZnO/CS/GCE对邻苯二酚的线性检测范围为1.00—47.00μmol·L^(-1),灵敏度56.35μA·(mmol·L^(-1))-1,检测限0.91μmol·L^(-1)(S/N=3),且电极具有良好的重复性和抗干扰性.Catechol has great harmful effects on the human body and environment.In this experiment,silver doped zinc oxide composites(Ag-ZnO)were prepared with electrospinning,and the tyrosinase/silver doped zinc oxide/chitosan/glassy carbon electrode(Tyr/Ag-ZnO/CS/GCE)were fabricated by loading tyrosinase(Tyr).The morphology,chemical composition,specific surface area of nanoparticles were characterized by scanning electron microscope(SEM),energy dispersire spectrometer(EDS),x-ray diffraction(XRD)and nitrogen adsorption-desorption.The cyclic voltammetry(CV)and electrochemical impedance spectroscopy(EIS)were used to test the electrochemical properties of Tyr/Ag-ZnO/CS/GCE electrodes,which ability to detect catechol(CA)was studied using chronoamperometry(IT).The results indicated that adding a small amount of Ag significantly reduced the particle size of Zn O,improved Zn O conductivity.Under optimal conditions,The fabricated Tyr/Ag-Zn O/CS/GCE exhibited a highly sensitive detection of CA with a wide linear range from 1.00μmol·L^(-1) to 47.00μmol·L^(-1),a sensitivity of 56.35μA·(mmol·L^(-1))-1 and a low detection limit of 0.91μmol·L^(-1)(S/N=3).Furthermore,the electrode has good repeatability and antiinterference.

关 键 词：银掺杂 氧化锌 酪氨酸酶 邻苯二酚 

分 类 号：X830[环境科学与工程—环境工程] O657.1[理学—分析化学]