不同炮制品组方的补阳还五汤抗脑缺血损伤及耐缺氧与抗疲劳作用比较研究  

Comparative Study on Anti-Cerebral is Chemia Injury,Anti-Hypoxia and Anti-Fatigue Effects of Buyang Huanwu Decoction with Different Preparation Formulations

作　　者：李卫先[1] 刘园园 李达[1] Li Weixian;Liu Yuanyuan;Li Da(Hunan College of Chinese Medicine,Zhuzhou 412012,China)

机构地区：[1]湖南中医药高等专科学校,湖南株洲412012

出　　处：《亚太传统医药》2022年第6期29-34,共6页Asia-Pacific Traditional Medicine

基　　金：湖南省中医药科研计划(2021145)。

摘　　要：目的:比较不同炮制品组方的补阳还五汤抗脑缺血损伤及耐缺氧与抗疲劳作用。方法:将大鼠随机分成7组,每组30只,即假手术组,模型组,脑心通组,不同炮制品组方的补阳还五汤1、2、3、4组;将小鼠分为6组,雌雄各半,每组65只,即空白对照组,脑心通组,不同炮制品组方的补阳还五汤1、2、3、4组。适应性饲养各组大鼠、小鼠各1周后,均参照动物与人体等效换算标准换算,给予相应的药物,补阳还五汤组按13g/(kg·d)量灌胃,脑心通胶囊组(0.4g/kg)水溶液灌胃,空白对照组、模型组与假手术组均给予等量生理盐水,均于造模手术前14d开始给药,持续至造模后第3天,每天2次。检测脑缺血后大鼠cAMP与β-EP、PF4、β-TG、血小板计数、大鼠白细胞计数、中性粒细胞计数及血浆纤维蛋白原含量及检测小鼠断头后喘气时间、钠石灰广口瓶中存活时间、恒温水浴中游泳被淹死的时间、小鼠夹闭气管后心电持续时间。结果:与假手术组比较,模型组大鼠血浆β-TG、PF4、β-EP、大鼠血小板计数及血浆纤维蛋白原含量、白细胞计数及中性粒细胞计数均显著升高,AMP含量显著降低(P<0.01)。与模型组比较,不同炮制品组方的补阳还五汤1、2、3、4,脑心通胶囊组均能不同程度降低大鼠血浆β-TG、PF4、β-EP含量及大鼠血小板计数和血浆纤维蛋白原含量、白细胞计数及中性粒细胞计数(P<0.05),且2、3、4、脑心通胶囊组均强于1组(各组方饮片均为生品)(P<0.05),其中以2组效果强于3、4、脑心通胶囊组(P<0.05),3组与4组、脑心通胶囊组比较差异无统计学意义(P>0.05);与空白组比较,各组灌胃给予不同炮制品组方的补阳还五汤2周后,小鼠断头后张口喘气时间均能不同程度延长(P<0.05),小鼠耐缺氧时间及游泳试验死亡时间均不同程度延长(P<0.05),均能不同程度延长夹闭气管后小鼠心电图持续时间(P>0.05)。结论:补阳还五汤临�Objective:To compare the anti-cerebral ischemia injury,anti-hypoxia and anti-fatigue effects of Buyanghuanwu decoction of different processed products.Methods:The rats were randomly divided into 7groups,each group of 30,namely the control group,model group,the heart failure group and the characteristics of different processed products of Yang also five soup group 1,2,3,4,divided the mice into 6groups,male and female 50,65in each group,namely blank control group,the heart failure group and the characteristics of different processed products of Yang also five soup group 1,2,3,4.After 1week of adaptive feeding,rats and mice in each group were given corresponding drugs according to the equivalent conversion standard between animal and human body.Buyanghuanwu decoction group was given intragastric administration at 13g/(kg·d),Naoxintong capsule group was given intragastric administration with water solution(0.4g/kg),blank control group,model group and sham operation group were given the same amount of normal saline.All the drugs were given on the 14th day before the modeling operation and continued until the 3rd day after modeling,twice a day.The cAMP,β-EP,PF4,β-TG,platelet count,white blood cell count,neutrophils count and plasma fibrinogen content of rats after cerebral ischemia were detected.The panting time,survival time in sodium and lime jar,drowning time in constant temperature water bath,and electrocardiogram duration after trachea clipping were also detected.Results:Compared with sham operation group,the plasmaβ-TG,PF4,β-EP,platelet count,plasma fibrinogen content,white blood cell count and neutrophil count in model group were significantly increased,while the AMP content was significantly decreased(P<0.01).Compared with model group,the contents ofβ-TG,PF4andβ-EP in plasma,platelet count,fibrinogen content in plasma,white blood cell count and neutrophils count in different processing groups of Buyang Huanwu Decoction 1,2,3,4and Naoxintong capsule groups could be decreased in different degree(P<0.05).Th

关 键 词：补阳还五汤 抗脑缺血损伤 抗耐缺氧与抗疲劳 不同炮制品组方 

分 类 号：R255.2[医药卫生—中医内科学] R285.5[医药卫生—中医学]