纤维素基固定化酶载体的研究进展  

Research progress of cellulose-based carrier for enzyme immobilization

作　　者：刘飞翔 董其惠 吴蓉 邱益彬 黄燕燕 苏二正 LIU Feixiang;DONG Qihui;WU Rong;QIU Yibin;HUANG Yanyan;SU Erzheng(Department of Food Science and Technology,College of Light Industry and Food Engineering,Nanjing Forestry University,Nanjing 210037,China;Department of Biological and Food Engineering,Bozhou University,Bozhou 236800,China;Co-Innovation Center for the Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,China;Co-Innovation Center for Efficient Processing and Utilization of Forest Products,Nanjing Forestry University,Nanjing 210037,China)

机构地区：[1]南京林业大学轻工与食品学院食品科学与工程系,江苏南京210037 [2]亳州学院生物与食品工程系,安徽亳州236800 [3]南京林业大学南方现代林业协同创新中心,江苏南京210037 [4]南京林业大学林业资源高效加工利用协同创新中心,江苏南京210037

出　　处：《生物加工过程》2022年第3期235-243,共9页Chinese Journal of Bioprocess Engineering

基　　金：江苏省“六大人才高峰”项目(2015-JY-016);中国博士后科学基金(2016M600417、2017T100373);江苏省第五期“333工程”项目(BRA2017458)。

摘　　要：固定化酶是工业生物催化中广泛应用的一种生物催化剂形式。作为固定化酶最重要的一个组成部分,载体材料的种类、结构和性能都会对酶的固定化效果产生重要影响,是成功制备固定化酶的关键。近年来,纤维素因具有可再生、可降解和廉价等优良特性而成为一种备受关注的固定化酶载体材料。纤维素基载体可以通过吸附、交联、共价结合以及包埋等方式实现酶的固定化,从而提高酶的热稳定性、酸碱耐受性、贮藏稳定性及其重复使用性。纤维素独特的结构及其可修饰性赋予纤维素基载体许多新的功能,如果以此作为酶固定化载体时,能够使固定化效率或固定工艺有更多的变化。本文综述改性纤维素、纤维素膜、纤维素小球等载体在酶固定化领域的研究进展、催化机制及应用,为相关的研究者提供参考。Immobilized enzyme is extensively used for industrial biocatalysis.As the most important component for enzyme immobilization,the type,structure and performance of carrier materials will have important effects on the immobilization of enzyme.Cellulose shows many excellent characteristics such as renewability,biodegradability,and financial feasibility.In recent years,cellulose used as the carrier for enzyme immobilization has garnered particular attention.The immobilization of enzymes can be realized by adsorption,cross-linking,covalent binding and embedding in the form of cellulose carrier.Most of the enzymes immobilized with cellulose as carrier materials show ameliorative thermal stability,acid and alkali tolerance,storage stability,and reusability.In this paper,we review the research progress on modified cellulose,cellulose membrane,cellulose beads and other cellulose forms used as carriers for enzyme immobilization.

关 键 词：酶 固定化 载体 纤维素 生物催化 生物材料 

分 类 号：Q814.2[生物学—生物工程]