Reprogramming of phytopathogen transcriptome by a non-bactericidal pesticide residue alleviates its virulence in rice  

作　　者：Haruna Matsumoto Yuan Qian Xiaoyan Fan Sunlu Chen Yanxia Nie Kun Qiao Dandan Xiang Xinzhong Zhang Meng Li Bo Guo Peilin Shen Qiangwei Wang Yunlong Yu Tomislav Cemava Mengcen Wang 

机构地区：[1]State Key Laboratory of Rice Biology&Ministry of Agricultural and Rural Affairs Laboratory of Molecular Biology of Crop Pathogens and Insects,Institute of Pesticide and Environmental Toxicology,Zhejiang University,Hangzhou 310058,China [2]Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China [3]Institute of Environmental Biotechnology,Graz University of Technology,Petersgasse 12,8010 Graz,Austria [4]Global Education Program for AgriScience Frontiers,Graduate School of Agriculture,Hokkaido University,Sapporo 0608589,Japan [5]State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China [6]Ecology and Environmental Sciences Center,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China [7]Key laboratory of South Subtropical Fruit Biology and Genetic Resource Uilization(MOA),Instiute of Fruit Tree Research,Guangdong Acadermy of Agricultural Sciences,Guangzhou 510640,China [8]Tea Research Instiute,Chinese Academy of Agriculural Sciences,Hangzhou 310008,China [9]Shanghai Intermnational Studies University,Shanghai 200083,China [10]Xiaoshan Agricultural Comprehensive Development Zone&Management Committee,Hangzhou 311200,China

出　　处：《Fundamental Research》2022年第2期198-207,共10页自然科学基础研究（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Grants No.32122074,31501684);National Key Research and Development Program of China(Grants No.2021YFE0113700,2017YFD0202100,2017YFE0102200);Zhejiang Provincial Natural Science Foundation of China(Grant No.LQ16C140001);Zhejiang Provincial Key Research and Development Program of China(Grant No.2015C02019);Strategic Research on“Plant Microbiome and Agroecosystem Health”(Grant No.2020ZL008;Cao Guangbiao High Science and Technology Foundation,Zhejiang University);the Fundamental Research Funds for the Central Universities(Grant No.2021FZZX001-31,Zhejiang University).

摘　　要：Bacteria equipped with virulence systems based on highly bioactive small molecules can circumvent their host's defense mechanisms.Pathogens employing this strategy are currently threatening global rice production.In the present study,variations in the virulence of the highly destructive Barkholderiaplantarii were observed in different rice-producing regions.The environment-linked variation was not attributable to any known host-related or external factors.Co-occurrence analyses indicated a connection between reduced virulence and 5-Amino-l,3,4-thiadiazole-2-thiol(ATT),a non-bactericidal organic compound.ATT,which accumulates in rice plants during metabolization of specific agrochemicals,was found to reduce virulence factor secretion by B.plantarii up to 88.8%and inhibit pathogen virulence by hijacking an upstream signaling cascade.Detailed assessment of the newly discovered virulence inhibitor resulted in mechanistic insights into positive effects of ATT accumulation in plant tissues.Mechanisms of virulence alleviation were deciphered by integrating high-throughput data,gene knockout mutants,and molecular interaction assays.TroK,a histidine protein kinase in a two-component system that regulates virulence factor secretion,is likely the molecular target antagonized by ATT.Our findings provide novel insights into virulence modulation in an important plant-pathogen system that relies on the host's metabolic activity and subsequent signaling interference.

关 键 词：PHYTOPATHOGEN Virulence factor Transcriptome reprogramming Agrochemical Pesticide RICE 

分 类 号：S51[农业科学—作物学]