丹参素对膀胱癌5637细胞Src/FAK信号通路及上皮间质转化的影响  

Effects of Danshensu on Src/FAK Signaling Pathway and Epithelial-Mesenchymal Transition in Bladder Cancer 5637 Cells

作　　者：罗仁修 高宏[2] 崔大为[2] 吕晓磊 LUO Renxiu;GAO Hong;CUI Dawei;LYU Xiaolei(Dept.of Urology Surgery,Tangshan Fengnan District Hospital,Hebei Tangshan 063300,China;Dept.of Urology Surgery,Tangshan Workers’Hospital,Hebei Tangshan 063000,China)

机构地区：[1]唐山市丰南区医院泌尿外科,河北唐山063300 [2]唐山市工人医院泌尿外科,河北唐山063000

出　　处：《中国医院用药评价与分析》2022年第5期584-587,共4页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：河北省医学科学研究课题计划项目(No.20201443)。

摘　　要：目的:探讨丹参素(Dss)对膀胱癌5637细胞类固醇受体共激活因子/黏着斑激酶(Src/FAK)信号通路及上皮间质转化(EMT)的影响。方法:用不同浓度的Dss(0、40、60和80μg/mL)处理膀胱癌5637细胞24 h后,分别命名为Dss-0组、Dss-40组、Dss-60组和Dss-80组;采用实时荧光定量聚合酶链反应检测膀胱癌5637细胞中EMT标志基因[E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)和波形蛋白(Vimentin)的信使核糖核酸(mRNA)]表达水平,采用蛋白质印迹法检测膀胱癌5637细胞中Src/FAK信号通路相关蛋白表达水平,采用细胞计数试剂盒8(CCK-8)法检测膀胱癌5637细胞增殖情况,采用划痕实验检测膀胱癌5637细胞的迁移能力。结果:Dss-0组、Dss-40组、Dss-60组和Dss-80组膀胱癌5637细胞E-cadherin mRNA表达水平依次升高,N-cadherin mRNA、Vimentin mRNA、磷酸化Src(p-Src)/Src蛋白、磷酸化FAK(p-FAK)/FAK蛋白表达水平以及吸光度、迁移率依次降低,呈浓度依赖性(P<0.05)。结论:Dss可能通过抑制Src/FAK信号通路来阻碍膀胱癌5637细胞EMT过程,进而抑制膀胱癌5637细胞增殖和迁移。OBJECTIVE: To probe into the effects of Danshensu(Dss) on steroid receptor coactivator/focal adhesion kinase(Src/FAK) signaling pathway and epithelial-mesenchymal transition(EMT) in bladder cancer 5637 cells. METHODS: Bladder cancer 5637 cells were treated with different concentrations of Dss(0 μg/mL, 40 μg/mL, 60 μg/mL and 80 μg/mL) for 24 h, and were named Dss-0 group, Dss-40 group, Dss-60 group and Dss-80 group, respectively. Real time fluorescence quantitative polymerase chain reaction was used to detect the mRNA expression level of EMT marker genes [E-cadherin, N-cadherin, Vimentin messenger RNA(mRNA)] in bladder cancer 5637 cells. Western blot was used to detect the expression level of related proteins in the Src/FAK signaling pathway in bladder cancer 5637 cells. Cell count kit 8(CCK-8) was used to detect the proliferation of bladder cancer 5637 cells. The scratch test was used to detect bladder cancer 5637 cells migration. RESULTS: In Dss-0 group, Dss-40 group, Dss-60 group and Dss-80 group, the expression level of E-cadherin mRNA in bladder cancer 5637 cells increased in sequence, N-cadherin mRNA, Vimentin mRNA, phosphorylated Src(p-Src)/Src protein, phosphorylated FAK(p-FAK)/FAK protein expression levels, absorbance(OD) and migration rate decreased in a concentration dependent manner(P<0.05). CONCLUSIONS: Dss may inhibit the EMT process of bladder cancer 5637 cells by inhibiting the Src/FAK signaling pathway, thereby inhibiting the proliferation and migration of bladder cancer 5637 cells.

关 键 词：丹参素 膀胱癌5637细胞 类固醇受体共激活因子/黏着斑激酶 上皮间质转化 

分 类 号：R965[医药卫生—药理学]