乌头内生细菌组成分析  

Composition of Endophytic Bacteria in Aconitum carmichaelii Debx.

作　　者：涂然 黄文峰 冯正平[2] 王娜[2] 赵阳[3] 崔浪军[2] TU Ran;HUANG Wen-fenga;FENG Zheng-ping;WANG Na;ZHAO Yang;CUI Lang-jun(Jingmen No.2 People's Hospital,Jingmen 448000,China;National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest China/The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry/The Ministry of Education,College of Life Sciences,Shaanxi Normal University,Xi'an 710062,China;Office of Pharmaceutical Clinical Research Institute,the Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004,China)

机构地区：[1]荆门市第二人民医院,湖北荆门448000 [2]西北濒危药材资源开发国家工程实验室/药用资源与天然药物化学教育部重点实验室/陕西师范大学生命科学学院,陕西西安710062 [3]西安交通大学第二附属医院药物临床研究机构办公室,陕西西安710004

出　　处：《中国现代中药》2022年第5期784-791,共8页Modern Chinese Medicine

基　　金：科技部科技惠民计划项目(2012GS610102);湖北省卫生计生委中医药科研项目(ZY2019Q035);中央高校创新团队项目(GK202001006);陕西省重点研发计划项目(2019ZDLSF04-01-01,2022SF-591)。

摘　　要：目的:揭示乌头内生细菌的菌群组成及其与生物碱含量的相关性。方法:运用高通量测序分析不同产地乌头叶、茎和根组织内生细菌的16S rDNA V4区,基于测序结果进行内生细菌的多样性分析、物种分类、差异及相似性分析。运用高效液相色谱法分析不同产地乌头叶、茎和根组织乌头类生物碱含量,并对乌头各组织的内生细菌相对丰度和生物碱含量之间进行相关性分析。结果:乌头内生细菌主要由4门,9纲,16目,36属组成;不同产地乌头的内生细菌丰度和多样性均存在差异,但核心菌群组成相似,以变形菌门(Proteobacteria)占主导;共发现6种操作分类单元(operational taxonomic units,OTU)与乌头根组织生物碱含量之间存在相关性。结论:不同产地乌头内生细菌组成存在差异但核心菌群相似,部分内生细菌丰度与生物碱含量之间显著相关。Objective:To reveal the composition of endophytic bacteria in Aconitum carmichaelii Debx.and the correlation with alkaloid content.Methods:The high-throughput sequencing was used to analyze 16S rDNA V4 regions of endophytic bacteria in the leaf,stem,and root tissue of A.carmichaelii Debx.,and the diversity analysis,species classification,and different and similar analysis of endophytic bacteria were conducted based on the sequencing results.Alkaloid content in leaf,stem,and root tissue of A.carmichaelii Debx.were detected by high performance liquid chromatography.Correlation between the relative abundance of endophytic bacteria and the alkaloid content in different tissues of A.carmichaelii Debx.was analyzed.Results:Endophytic bacteria of A.carmichaelii Debx.were mainly composed of 4 phyla,9 classes,16 orders,and 36 genera.The abundance and diversity of endophytic bacteria in A.carmichaelii Debx.were different,but the composition of core flora was similar with Proteobacteria predominating.Six operational taxonomic units(OTU)were found correlated with alkaloid content in root tissues of A.carmichaelii Debx..Conclusion:The composition of endophytic bacteria in A.carmichaelii Debx.From different regions was different,but the core flora was similar.The abundance of partial endophytic bacteria and alkaloid content were significantly correlated.

关 键 词：乌头 内生细菌 高效液相色谱 乌头碱 相关性分析 

分 类 号：R284[医药卫生—中药学]