五味子药材提取工艺优化及8个木脂素类成分含量测定  

Optimization of Extraction Process and Determination of Eight Effective Components in Schisandrae Chinensis Fructus

作　　者：朱琳 侯丽华 刘亮亮 陈素娥 ZHU Lin;HOU Li-hua;LIU Liang-liang;CHEN Su-e(Shanxi Health Vocational College,Jinzhong 030619,China)

机构地区：[1]山西卫生健康职业学院,山西晋中030619

出　　处：《中国现代中药》2022年第5期861-867,共7页Modern Chinese Medicine

基　　金：山西省重点研发计划项目(201903D321213)。

摘　　要：目的:优化木脂素类成分的提取方法,并建立高效液相色谱法(HPLC)测定五味子药材中8个木脂素类成分(五味子醇甲、五味子醇乙、当归酰戈米辛H、五味子酯甲、五味子酯乙、五味子甲素、五味子乙素和五味子丙素)的含量测定方法。方法:采用Box-Behnken响应面法,以超声时间、甲醇体积分数、料液比为考察因素,以8个木脂素类成分总含量为考察指标来优化提取工艺,并验证此工艺。采用AgilentZB-ExtendC_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为0.5%磷酸水溶液-乙腈,梯度洗脱,流速为1 mL·min^(-1),检测波长为220 nm,柱温为35℃。结果:五味子药材中木脂素类成分的最优提取工艺为超声时间30 min,甲醇体积分数为100%,料液比1∶50;不同产地五味子的8个木脂素类成分含量有差异,RSD为8.44%~20.02%。结论:Box-Behnken响应面优化五味子中木脂素的提取工艺科学稳定、准确可靠,建立测定8个木脂素类成分含量的方法专属性强、重复性好、简便准确,可为五味子药材的质量评价及工业化提取生产提供参考。Objective:Optimize the extraction process and then to establish a high performance liquid chromatography(HPLC)method for determining the content of eight lignans(schisandrol A,schisandrol B,gomisin H,schisantherin A,schisantherin B,schisandrin A,schisandrin B,and schisandrin C).Methods:Box-Behnken design was used to optimize the extraction process.To be specific,the optimal sonication time,methanol concentration,and material-to-liquid ratio in the extraction process were investigated with the content of the eight components as evaluation index.The optimal process was then validated.HPLC conditions are as follows:Agilent ZB-Extend C_(18) column(250 mm×4.6 mm,5μm),column temperature of 35℃,mobile phase of 0.5%phosphoric acid solution-acetonitrile for gradient elution at the flow rate of 1 mL·min^(-1),detection wavelength of 220 nm.Results:The optimal extraction process is as below:sonication for 30 min,100%methanol as solvent,and material-to-liquid ratio of 1∶50.The content of the 8 lignans in Schisandrae Chinensis Fructus of different producing areas was different,with the RSD of 8.44%-20.02%.Conclusion:The optimized extraction process is scientific,stable,accurate and reliable.The established method for determining the content of 8 components is highly specific,reproducible,simple and accurate.The result of this study can serve as a reference for quality evaluation and industrial extraction of Schisandrae Chinensis Fructus.

关 键 词：五味子 木脂素 含量测定 Box-Behnken响应面法 提取工艺 

分 类 号：R282.71[医药卫生—中药学]