Difference Analysis of Liver Metabolic Response Between Diploid and Triploid Rainbow Trout Oncorhynchus mykiss Under Fishing Stress  

作　　者：Han Yue Lv Xiao-nan Chen Si-miao Zhou Qun Qin Mei-chuan Han Ying 

机构地区：[1]College of Animal Sciences and Technology,Northeast Agricultural University,Harbin 150030,China [2]Beijing Fisheries Technology Promotion Station,Beijing 100000,China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2022年第2期51-61,共11页东北农业大学学报（英文版）

基　　金：Supported by Heilongjiang Natural Science Foundation(C2017013);China Postdoctoral Science Foundation(2017M611347)。

摘　　要：To elucidate the effects of fishing stress on the liver glucose metabolism of both diploid and triploid female rainbow trouts as well as differences in their stress response,blood and liver tissues were collected from 0 h to 24 h after the fishing stress.Blood indexes,such as the levels of white blood cells and red blood cells,cortisol,glucose and lactic acid,as well as activities of key enzymes of the glucose metabolism in the liver,were measured.Furthermore,the mRNA expressions of glucocorticoid receptors and enzymes related to energy metabolism were assessed.The results showed that fishing stress exerted significant effects on blood physiological and biochemical indexes and liver glucose metabolism.Differences were found between diploid and triploid female rainbow trouts in the liver glucose metabolism pathway,as well as the level and performance during stress.The basic glucose metabolism intensity of the triploid liver was higher than that of the diploid liver;however,the hepatic glycogen reserve was lower in the triploid liver.After fishing stress,triploid trouts entered the immunosuppressive state earlier than diploid trouts.Triploid trouts also showed an earlier and stronger stress reaction than diploid trouts,while their energy allocation ability and immune recovery ability were weaker than those of diploid trouts.These results showed that the regulatory ability of diploid female rainbow trouts in response to fishing stress was better than that of triploid trouts.

关 键 词：rainbow trout POLYPLOID handing energy metabolism 

分 类 号：S943[农业科学—水产养殖]