变应性鼻炎患儿血清IL-33,ST2水平及IL-33基因rs3939286 G/A位点多态性与疾病程度相关性分析  

Correlation Analysis between the Levels of IL-33,ST2 and Polymorphism of IL-33 Gene rs3939286 G/A and Different Degree of Allergic Rhinitis in Children

作　　者：林李娜 何微 刘国栋 凌秀婷 王小青 梁玉玲 王晶晶 梁丕凤 LIN Li-na;HE Wei;LIU Guo-dong;LING Xiu-ting;WANG Xiao-qing;LIANG Yu-ling;WANG Jing-jing;LIANG Pi-feng(Department of General Clinic,Shenzhen Longhua District People’s Hospital,Guangdong Shenzhen 518109,China;Department of Clinical Laboratory,Shenzhen Longhua District People’s Hospital,Guangdong Shenzhen 518109,China)

机构地区：[1]深圳市龙华区人民医院全科门诊,广东深圳518109 [2]深圳市龙华区人民医院检验科,广东深圳518109

出　　处：《现代检验医学杂志》2022年第3期127-131,共5页Journal of Modern Laboratory Medicine

摘　　要：目的分析变应性鼻炎(allergic rhinitis,AR)患儿血清白细胞介素-33(interleukin-33,IL-33)、特异性受体肿瘤发生抑制蛋白2(specific receptor suppression of tumorigenicity 2,ST2)水平及IL-33基因rs3939286 G/A位点单核苷酸多态性(single nucleotide polymorphism,SNP)与疾病程度的相关性。方法收集2020年3月~2021年6月深圳市龙华区人民医院AR确诊患儿135例作为AR组,体检健康儿童116例作为对照组。分别检测血清IL-33和ST2水平,同时对IL-33基因rs3939286 G/A位点SNP进行分析。结果AR组IL-33和ST2水平(251.67±39.15 pg/ml,5.23±0.29 pg/ml)明显高于对照组(108.24±20.63 pg/ml,2.06±0.11 pg/ml),差异有统计学意义(t=7.3416,8.0197,均P<0.05)。中-重度AR患儿IL-33和ST2水平(374.95±58.32 pg/ml,7.98±0.54pg/ml)明显高于轻度AR(164.28±30.37 pg/ml,3.28±0.17 pg/ml),差异有统计学意义(t=7.6027,8.3150,均P<0.05)。AR组IL-33基因rs3939286 G/A位点AA基因型和A等位基因频率(55.56%,70.74%)明显高于对照组(24.14%,38.36%),差异有统计学意义(χ^(2)=6.2184,5.3092,均P<0.05)。中-重度AR患儿IL-33基因rs3939286 G/A位点AA基因型和A等位基因频率(73.42%,84.18%)明显高于轻度AR患儿(44.65%,62.50%),差异有统计学意义(χ^(2)=4.9023,3.9814,均P<0.05)。AA基因型AR患儿血清IL-33水平(303.12±41.60 pg/ml)明显高于AG基因型(214.38±33.15pg/ml),而AG基因型明显高于GG基因型(129.06±23.78pg/ml),不同基因型差异有统计学意义(χ^(2)=3.5054,P=0.0394)。结论AR患儿IL-33和ST2水平明显升高,与疾病程度有关。同时AR患儿IL-33基因rs3939286 G/A位点存在多态性,不同基因型IL-33水平存在明显差异,其中AA基因型IL-33水平最高,与AR患儿发病存在一定相关性。Objective To analyze the correlation of interleukin-33(IL-33)and specific receptor suppression of tumorigenicity 2(ST2)levels,single nucleotide polymorphism(SNP)of IL-33 gene rs3939286 G/A locus and allergic rhinitis(AR)in children with different degrees.Methods 135 children with AR in Shenzhen Longhua District People’s Hospital from March 2020 to June 2021 were selected as AR group,and 116 healthy children were selected as control group.The levels of IL-33 and ST2 in serum were detected,and SNP of IL-33 gene rs3939286 G/A locus was analyzed.Results The levels of IL-33 and ST2 in AR group(251.67±39.15pg/ml,5.23±0.29 pg/ml)were significantly higher than(108.24±20.63pg/ml,2.06±0.11pg/ml)in control group,the differences were statistically significant(t=7.3416,8.0197,all P<0.05).The levels of IL-33 and ST2 in moderatesevere AR children(374.95±58.32pg/ml,7.98±0.54pg/ml)were significantly higher than(164.28±30.37pg/ml,3.28±0.17pg/ml)in mild AR children,and the differences were statistically significant(t=7.6027,8.3150,all P<0.05).The AA genotype and A allele frequency of IL-33 gene rs3939286 G/A locus in AR group(55.56%,70.74%)were significantly higher than(24.14%,38.36%)in control group,the differences were statistically significant(χ^(2)=6.2184,5.3092,all P<0.05).The AA genotype and A allele frequency(73.42%,84.18%)of IL-33 gene rs3939286 G/A locus in children with moderate to severe AR were significantly higher than in children with mild AR(44.65%,62.50%),and the differences were statistically significant(χ^(2)=4.9023,3.9814,all P<0.05).The level of IL-33 in AR children with AA genotype(303.12±41.60pg/ml)was significantly higher than that of AG genotype(214.38±33.15pg/ml),while AG genotype was significantly higher than that of GG genotype(129.06±23.78pg/ml),and there were significant differences among different genotypes(χ^(2)=3.5054,P=0.0394).Conclusion The levels of IL-33 and ST2 in AR children are significantly increased,which was related to the severity of the disease.At the same time,there was p

关 键 词：白细胞介素-33 特异性受体肿瘤发生抑制蛋白2 单核苷酸多态性 变应性鼻炎 

分 类 号：R765.21[医药卫生—耳鼻咽喉科] R392.11[医药卫生—临床医学]