藏红花素通过调控微管蛋白进而影响胃癌细胞MGC-803的增殖、侵袭和凋亡  

Crocetin affects the proliferation,invasion and apoptosis of gastric cancer cell line MGC-803 by regulating tubulin Saffin affects the proliferation,invasion and apoptosis of gastric cancer cell line MGC-803 by regulating tubulin

作　　者：廖冰灵 周颖[1] 许琦华[1] 田天宁 常玲 张慧君 LIAO Bing-ling;ZHOU Ying;XU Qi-hua;TIAN Tian-ning;CHANG Ling;ZHANG Hui-jun(Department of Gastroenterology, Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China;Department of Emergency surgery, Seventh People’s Hospital of Shanghai University of Traditional Chinese)

机构地区：[1]上海市第七人民医院消化科,200137 [2]上海市第七人民医院急创中心急诊外科,200137

出　　处：《现代消化及介入诊疗》2022年第3期311-315,共5页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：浦东新区卫生健康委员会优秀青年医学人才培养计划(PWRq2020-23);上海中医药大学附属第七人民医院人才培养计划(XX2019-12)。

摘　　要：目的 探讨藏红花素(Crocin)是否通过调控微管蛋白进而影响人胃癌细胞MGC-803的增殖、侵袭和凋亡,以期为胃癌的治疗提供新的思路。方法使用藏红花素处理MGC-803细胞,并用顺铂作为阳性对照药,观察藏红花素对MGC-803细胞生物学的影响。采用MTT分析细胞活性,流式细胞术分析细胞凋亡,Transwell检测细胞的侵袭能力,划痕实验检测细胞的迁移能力。最后通过蛋白免疫印迹分析藏红花素作用的潜在机制。结果 藏红花素使MGC-803细胞活力、迁移和侵袭能力明显下降,凋亡增加。进一步研究发现,藏红花素对MGC-803细胞的抑制能力与微管蛋白作用密切相关,藏红花素通过降低MGC-803细胞中的微管蛋白表达进而影响其增殖、侵袭和凋亡。结论藏红花素能够显著抑制MGC-803细胞生长、迁移、侵袭能力,并能促进MGC-803胃癌细胞的凋亡反应。Objective To explore whether crocin affects the proliferation,invasion and apoptosis of human gastric cancer cells MGC-803 by regulating tubulin,in order to provide new ideas for the treatment of gastric cancer.Methods MGC-803 cells were treated with crocin,and cisplatin was used as a positive control drug to observe the effect of crocin on the biology of MGC-803 cells.MTT was used to analyze cell viability,flow cytometry was used to analyze cell apoptosis,Transwell was used to detect cell invasion ability,and scratch assay was used to detect cell migration ability.Finally,the underlying mechanism of the action of crocin was analyzed by Western blot.Results Crocin significantly decreased the viability,migration and invasion ability of MGC-803 cells,and increased apoptosis.Further research found that the inhibitory ability of crocin on MGC-803 cells was closely related to the effect of tubulin,and crocin affected the proliferation,invasion and apoptosis of MGC-803 cells by reducing the expression of tubulin in MGC-803 cells.ConclusionCrocin can significantly inhibit the growth,migration and invasion of MGC-803 cells,and promote the apoptotic response of MGC-803 gastric cancer cells.

关 键 词：藏红花素 微管蛋白 胃癌 增殖 

分 类 号：R285[医药卫生—中药学]