大孔树脂纯化细毡毛忍冬中总黄酮工艺研究  

Research on Techniques of Purifying the Total Flavonoids in Lonicera Similes Hemsl with Macroporous Resin

作　　者：郑光雅 ZHENG Guangya(Central Hospital of Sichuan Provincial Prison Administration,Chengdu 610000,China)

机构地区：[1]四川省监狱管理局中心医院,四川成都610000

出　　处：《中国民族民间医药》2022年第11期32-35,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：四川省教育厅重点项目课题(川银花主流品种细毡毛忍冬特征性成分的分离及含量测定研究,项目编号:10ZA085)。

摘　　要：目的:研究大孔树脂纯化细毡毛忍冬总黄酮的工艺,为细毡毛忍冬化学成分的进一步研究奠定基础。方法:以静态吸附容量及解析率筛选树脂类型,在动态吸附条件上,对影响纯化的工艺参数条件进行筛选。结果:确定了大孔树脂纯化细毡毛忍冬提取物的工艺为:D101大孔树脂纯化细毡毛忍冬提取物,上柱药液生药浓度为0.10 g/mL,比上柱量2.74g/g(药材/干树脂)上柱吸附。结论:D101树脂综合性能较好,适用于细毡毛忍冬中总黄酮成分的分离纯化。Objective To research on a purification process for the total flavonoids from Lonicera similes Hemsl by macmporous resin,and lay the foundation for the further research of the chemical composition of Lonicera similes Hemsl.Methods Based on the different static adsorption capacity and elution rate of total flavonoids,the technological conditions for absorption of macroporous absorptive resin were selected.Result The result indicates that the optimum purification process are as follows:D101 macroporous resin is used to purify the extract of Lonicera similes Hemsl,The sample solution concentration is 0.10 g crude drug/mL and the rate amount is 2.74 g/g crude drug dry resin.Conclusion With good comprehensive properties,D101 resin is suitable for purifying the element of total flavone from Lonicera similes Hemsl.

关 键 词：细毡毛忍冬 大孔树脂 总黄酮 纯化 

分 类 号：R284.2[医药卫生—中药学]