微针在皮肤间质液生物分析中的研究进展  

Research Progress of Microneedles in Interstitial Fluid Bioanalysis

作　　者：卢惠婷 李金泽 王夜宇 葛如娇 董海峰 LU Hui-Ting;LI Jin-Ze;WANG Ye-Yu;GE Ru-Jiao;DONG Hai-Feng(Beijing Key Laboratory for Bioengineering and Sensing Technology,School of Chemistry&Biological Engineering,University of Science&Technology Beijing,Beijing 100083,China;Marshall Laboratory of Biomedical Engineering,School of Biomedical Engineering,Shenzhen University,Shenzhen 518060,China)

机构地区：[1]北京科技大学化学与生物工程学院,生物工程与传感技术北京市重点实验室,北京100083 [2]深圳大学生物医学工程学院,马歇尔生物医学工程实验室,深圳518060

出　　处：《分析化学》2022年第6期819-829,共11页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21874008,22004006,22022407,21890740,21890742);国家重大研究计划专项基金项目(No.2019YFC1606603);深圳大学2021年稳定支持计划项目(No.8940206/0200)资助。

摘　　要：对生物样品进行采集并分析测定是疾病诊断和治疗的有效手段,其中,血液取样和分析是临床诊断中的标准方法,但血液采样具有损伤性,通常会导致皮肤组织损伤从而引起患者不适。皮肤间质液(Interstitial skin fluid,ISF)具有丰富的生物学信息,是一种很有前景的血液替代品,然而,以微创和非侵入性方式采集ISF中足够的标志物仍然具有很大的挑战。近年来,微针(Microneedles,MNs)技术因其具有高效、安全、方便和无痛等特点,在ISF研究中备受关注。MNs作为一种微米级别的针阵列,在医疗美容、分子递送、诊断监测以及疾病治疗等方面取得了显著的成果,但在ISF生物分析中的应用很少。本文总结了制备MNs的材料,详细描述了MNs在ISF采样、生物标志物捕获和分析以及可穿戴传感等方面的应用,展望了MNs作为一个新兴研究领域的发展前景,并总结了在技术成果转化过程中需要考虑和存在的问题。The collection and analysis of biological samples is the effective method for disease diagnosis and treatment. Blood sampling and analysis are standard methods in clinical diagnosis, but they are commonly invasive and usually cause tissue damage and patient discomfort. Interstitial fluid(ISF)is a promising alternative candidate of blood owing to the rich biological information;however,it is still great challenge to minimally and non-invasively sample sufficient targets in skin ISF. In recent years, microneedles(MNs)have received considerable attention in ISF research due to their high efficiency, safety, convenience and painless characteristics. As the micron-level needle array, MNs have achieved remarkable results in medical cosmetology, molecular delivery, diagnostic monitoring and disease treatment, but little attention has been paid to the application of MNs for bioanalysis in ISF. This review summarizes the materials of microneedle, and details the application of MNs in ISF sampling,biomarker capture and analysis, and wearable sensing, while exploring the development prospects of MNs as an emerging research field and summarizing the challenges to be considered and existing in the transformation of technological achievements.

关 键 词：微针 生物分析 体液采样 微创 评述 

分 类 号：R446.1[医药卫生—诊断学]