74个观赏海棠品种的遗传多样性及指纹图谱构建  

Fingerprints Establisment and Genetic Diversity of 74 Ornamental Crabapple Varieties

作　　者：董聚苗 刘月 李扬森 冯树香 程蓓蓓 刘振林[1] 张国君[1] DONG Jumiao;LIU Yue;LI Yangsen;FENG Shuxiang;CHENG Beibei;LIU Zhenlin;ZHANG Guojun(College of Horticulture Science and Technology/Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization,Hebei Normal University of Science&Technology,Changli Hebei 066600,China;Tangshan Abundant South Area of Agricultural Technical High School,Tangshan Hebei 063306,China;Yongqing Agricultural and Rural Bureau,Yongqing Hebei 065699,China;Hebei Academy of Forestry and Grassland Sciences/Hebei Forest City Construction Technology Innovation Center,Shijiazhuang 050061,China)

机构地区：[1]河北科技师范学院园艺科技学院/河北省特色园艺种质挖掘与创新利用重点实验室,河北昌黎066000 [2]唐山市丰南区农业技术高级中学,河北唐山063306 [3]永清县农业农村局,河北永清065699 [4]河北省林业和草原科学研究院/河北省森林城市建设技术创新中心,石家庄050061

出　　处：《种子》2022年第5期30-35,50,共7页Seed

基　　金：河北省重点研发计划项目(21326301 D);河北省研究生创新资助项目(CXZZSS 2021146);河北省高校园艺作物育种应用技术研发中心项目(YF 201404);河北省级专业学位研究生培养实践基地(唐山植物园,202018)。

摘　　要：观赏海棠种质资源丰富,表型鉴定与分类难度大。本试验以74个观赏海棠品种为材料,利用SSR分子标记以及表型性状测定,分析观赏海棠的遗传多样性并构建指纹图谱。利用20对谱带清晰、多态性好的引物对74个种(品种)进行PCR扩增及遗传多样性分析,共检测到766个等位基因,扩增出的多态位点百分率达100%,每个位点的平均等位基因数为38.3个。遗传多样性信息含量变化幅度为0.86~0.97,基因多样性指数平均值为0.94,表现出丰富的遗传多样性。从分子水平上探讨其亲缘关系,基于SSR的聚类结果可将74个观赏海棠品种分为四大类。最终利用3对SSR引物成功地构建了74个观赏海棠的指纹图谱,为海棠种质资源的保护、开发利用及创新提供参考和依据。It is difficult to identify and classify from phenotype as ornamental crabapple is rich in germplasm resources.Genetic diversity of 74 varieties was analysed using SSR molecular markers and phenotypic traits,and their fingerprints were constructed.PCR amplification and genetic diversity analysis of 74 species(variety)was carried out with 20 pairs of primers of clear bands and good polymorphism.A total of 766 alleles were detected,and the percentage of amplified polymorphic loci reached 100%,the average number of alleles per locus was 38.3.The variation range of polymorphism information content was 0.86-0.97.The average value of gene diversity was 0.94,which showed abundant genetic diversity.Based on SSR clustering results by exploring their kinships at the molecular level,74 ornamental crabapple varieties coud be divided into four categories.The fingerprints of 74 orna mental crabapple varities were successfully constructed using 3 pairs of SSR primers.It would provide reference and basis for the protection,development,utilization and innovation of ornamental crabapple germplasm resources.

关 键 词：观赏海棠 SSR分子标记 指纹图谱 遗传多样性 

分 类 号：S722.3[农业科学—林木遗传育种] S686[农业科学—林学]