北苍术根腐病病原鉴定及生物学特性研究  

Identification and Biological Characteristics of the Pathogen Causing Root Rot of Atractylodes chinensis

作　　者：霍佳欢 温晓蕾 李双民[3] 冯丽娜 兰淑慧 董立新 郭思柔 栗佳宁 王建华 齐慧霞 HUO Jiahuan;WEN Xiaolei;LI Shuangmin;FENG Lina;LAN Shuhui;DONG Lixin;GUO Sirou;LI Jianing;WANG Jianhua;QI Huixia(Hebei Key Laboratory of Crop Stress Biology,College of Agronomy and Biotechnology,Hebei Normal University of Science&Technology,Hebei Qinhuangdao 066600,China;College of Plant Protection,Hebei Agricultural University,Hebei Baoding 071001,China;Hebei Changli vocational and Technical Education Center,Hebei Changli 066600,China;Qinhuangdao Plant Protection and Inspection Station,Hebei Qinhuangdao 066600,China)

机构地区：[1]河北科技师范学院农学与生物科技学院,河北省作物逆境生物学重点实验室,河北秦皇岛066600 [2]河北农业大学植物保护学院,河北保定071002 [3]河北省昌黎县职业技术教育中心,河北秦皇岛066600 [4]秦皇岛市植保植检站,河北秦皇岛066000

出　　处：《中国农业科技导报》2022年第5期137-144,共8页Journal of Agricultural Science and Technology

摘　　要：根腐病严重影响北苍术生产,育苗期至成株期均有发生。为明确引起河北省秦皇岛地区北苍术根腐病病原菌,采用组织分离法获得致病菌菌株,并通过形态学及分子生物学相结合的方法对其进行鉴定,同时分析温度、氮源、碳源对病原菌生长的影响以及菌丝的致死温度。结果表明,引起秦皇岛地区北苍术根腐病的病原菌为尖孢镰刀菌Fusarium oxysporum(MK849925),该病原菌在PDA培养基上菌落近圆形,呈辐射状生长,似绒毛,粉紫色,较干燥。菌丝侧生分支,有隔膜;分生孢子均无色,大型分生孢子呈渐尖型镰刀状,有1~5个分隔,小型分生孢子多为卵形,具1~2个分隔,厚垣孢子圆形、无色。其致病力较强,接种后10 d病部呈深褐色、失水干枯。该病原菌较适宜的生长条件为25℃、以酵母浸粉为基础氮源的培养基,培养5 d,菌落直径可达7.17 cm;在不同碳源中,对葡萄糖和蔗糖的利用率较高,而对于可溶性淀粉的利用率较低。菌丝在75℃恒温条件下处理10 min后失去生命力。Root rot seriously affects the production of Atractylodes chinensi,which occurrs from seedling to mature periods.In order to clarify the pathogen causing Atractylodis chinensi root rot in Qinhuangdao area of Hebei Province,tissue isolation,morphological and molecular biological identification were used to isolate and identify the pathogen,and the pathogenicity was identified.At the same time,the effects of temperature,nitrogen source and carbon source on the growth of the pathogen and the lethal temperature of the mycelium were analyzed.The results showed that the pathogen causing root rot of Atractylodis chinensi in Qinhuangdao area was Fusarium oxysporum(MK849925).The colony of the pathogen on PDA medium was nearly round,radial,villous,pink purple and dry.Mycelium lateral branched with septum,the conidia were all colorless and the large conidia were acuminate sickle with 1~5 septations,the small conidia were mostly oval with 1~2 septations.The chlamydospores were round and colorless.Its pathogenicity was strong,and the disease showed dark brown dehydration dry after inoculation for 10 d.The pathogen grew better at 25℃and on the medium with yeast extract powder as the nitrogen source.After 5 d culture,the colony diameter reached 7.17 cm.Among different carbon sources,the utilization rates of glucose and sucrose were high,while the utilization rate of soluble starch was low.The mycelium lost its vitality after treated at 75℃for 10 min.

关 键 词：北苍术 根腐病 尖孢镰刀菌 病原鉴定 生物学特性 

分 类 号：S432[农业科学—植物病理学]