赤霉素生物合成抑制剂促进杧果成花的作用和对顶芽代谢谱的影响  

Effects of gibberellin biosynthesis inhibitor on floral induction and apical bud metabolic profiles in mango

作　　者：梁飞 许文天 武红霞[2] 郑斌[2] 梁清志[2] 王松标[2] 李映志[1] LIANG Fei;XU Wentian;WU Hongxia;ZHENG Bin;LIANG Qingzhi;WANG Songbiao;LI Yingzhi(Binhai Agricultural College of Guangdong Ocean University,Zhanjiang 524088,Guangdong,China;South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Tropical Fruit Biology of Ministry of Agriculture and Rural Affairs,Zhanjiang 524091,Guangdong,China)

机构地区：[1]广东海洋大学滨海农业学院,广东湛江254088 [2]中国热带农业科学院南亚热带作物研究所·农业农村部热带果树生物学重点实验室,广东湛江524091

出　　处：《果树学报》2022年第6期957-969,共13页Journal of Fruit Science

基　　金：海南省自然科学基金项目(320QN323);国家重点研发计划项目(2019YFD1000500,2020YFD1000604);广东省现代农业产业技术创新团队专项基金项目(2019KJ108);广东海洋大学创新强校项目(GDOU2013050217,GDOU2016050256)。

摘　　要：【目的】探明赤霉素合成抑制剂缩节胺、调环酸钙和烯效唑组合物(简称SPD)诱导杧果成花效应机制。【方法】利用超高效液相色谱串联质谱(UPLC-MS/MS)的广泛靶向代谢组学研究方法,分析杧果成花诱导不同阶段顶芽代谢物差异。【结果】与清水处理相比,应用SPD处理,检测到的582种代谢物中有372种存在差异(变量重要性投影VIP≥1,差异倍数FC≥2或≤0.5)。其中,在SPD处理80~100 d后,随着顶芽的休眠解除,顶芽中脂质、酚酸、氨基酸、碳水化合物和维生素等代谢物含量随着时间推移发生了显著的变化。18种脂质包括12种溶血磷脂酰乙醇胺(LPE)、7种溶血磷脂酰胆碱(LPC)和1种游离脂肪酸(FA)在80~100 d显著上调,脯氨酸、抗坏血酸、碳水化合物和单宁含量持续增加。而L-半胱氨酸、L-组氨酸和L-高甲硫氨酸,在SPD处理30~100 d后相对含量变化超过10倍,但在对照组中没有显著变化。【结论】研究结果为亚热带地区杧果开花管理提供一种潜在的方法,并为应用赤霉素生物合成抑制剂促进杧果成花内在机制的解析提供理论依据。【Objective】Mango(Mangifera indica L.)is one of the most important fruit crops in the tropical and subtropical regions,and China is the second-largest producer of mangoes after India.Floral induction(FI)is an important development event in perennial woody fruit trees,which determines the onset of fruit and plays a crucial role in yield.Mango FI starts from dormant bud or growing bud and ends with the beginning of flower bud morphological differentiation(commonly known as brush head in production).Although the mechanisms that control flowering remain primarily unknown in mango,it can be induced by cold temperatures.However,insufficient chilling in winter due to the aggravation of global warming has affected mango FI and results in decreased fruit production of commercial mango orchards in China,mainly in Hainan,Guangdong and Guangxi.In recent decades,soil drenching of paclobutrazol(PBZ)has been used as a major practice to induce mango flowering in many commercial mango orchards,including Guangdong,Guangxi and Hainan in China.PBZ inhibits gibberellin biosynthesis and vegetative growth,and thus enhances mango FI.However,inappropriate doses of PBZ over a long period can result in soil residues,new bud and panicle compaction,and increased incidence of disease.In addition to PBZ,there are several safer gibberellin inhibitors such as uniconazole,prohexadione calcium and mepiquat chloride,which play roles in regulating plant growth.More importantly,they have the characteristics of low toxicity and low residue in soil.For example,the activity of uniconazole is 6-10 times higher than that of paclobutrazol,but its residue in soil is only 1/10 of that of paclobutrazol.Prohexadione calcium can be rapidly degraded into water and carbon dioxide by microorganisms in the soil,and has no residual toxicity to rotation plants and no pollution to the environment,whereas,the effects of these agents on mango flowering regulation have not been evaluated.This research aimed to carry out a comprehensive analysis of the mechanism of man

关 键 词：杧果 成花诱导 代谢谱 脂质 氨基酸 

分 类 号：S667.7[农业科学—果树学]