Determining the protective effects of Ma-Mu-Ran Antidiarrheal Capsules against acute DSS-induced enteritis using 16S rRNA gene sequencing and fecal metabolomics  

作　　者：ZHENG Si-Li ZHANG Dong-Ning DU AN Yan-Fen HUANG Fang HAN Lin-Tao MO Guo-Yan 

机构地区：[1]Pharmacy Faculty,Hubei University of Chinese Medicine,Wuhan 430065,China [2]College of Basic Medical Sciences,Hubei University of Chinese Medicine,Wuhan 430065,China [3]Xinjiang Uygur Pharmaceutical Co.,Ltd.,Urumqi,Xinjiang 830026,China

出　　处：《Chinese Journal of Natural Medicines》2022年第5期364-377,共14页中国天然药物（英文版）

基　　金：supported by Tianchi plan of introducing high-level talents in the Xinjiang Uygur Autonomous Region(2019-39);Major projects of Xinjiang Uygur Autonomous Region“Research and development of new ethnic drugs and large varieties”(2016A03005-2).

摘　　要：Ma-Mu-Ran Antidiarrheal Capsules(MMRAC)is traditional Chinese medicine that has been used to treat diarrhea caused by acute enteritis(AE)and bacillary dysentery in Xinjiang(China)for many years.However,the potential therapeutic mechanism of MMRAC for AE and its regulatory mechanism on host metabolism is unclear.This study used fecal metabolomics profiling with GC/MS and 16S rRNA gene sequencing analysis to explore the potential regulatory mechanisms of MMRAC on a dextran sulfate sodium salt(DSS)-induced mouse model of AE.Fecal metabolomics-based analyses were performed to detect the differentially expressed metabolites and metabolic pathways.The 16S rRNA gene sequencing analysis was used to assess the altered gut microbes at the genus level and for functional prediction.Moreover,Pearson correlation analysis was used to integrate differentially expressed metabolites and altered bacterial genera.The results revealed that six intestinal bacteria and seven metabolites mediated metabolic disorders(i.e.,metabolism of amino acid,carbohydrate,cofactors and vitamins,and lipid)in AE mice.Besides,ten altered microbes mediated the differential expression of eight metabolites and regulated these metabolisms after MMRAC administration.Overall,these findings demonstrate that AE is associated with metabolic disorders and microbial dysbiosis.Further,we present that MMRAC exerts protective effects against AE by improving host metabolism through the intestinal flora.

关 键 词：Acute enteritis Ma-Mu-Ran Antidiarrheal Capsules 16S rRNA sequencing Fecal metabolomics Gut microbiota 

分 类 号：R96[医药卫生—药理学] R516.1[医药卫生—药学]