背根神经节P2X3R和TRPV1介导CFA致炎性痛大鼠不同时期痛行为  

Differential roles of P2X3R and TRPV1 in dorsal root ganglion contributed to pain behavior at diverse phases in rats with inflammatory pain

作　　者：吴茗慧 甄思佳 徐婷[1] 周游 应帅宇 叶思婷 杜俊英[1] 房军帆[1] 方剑乔[1,2] 高宏[2] 梁宜[1,2] WU Minghui;ZHEN Sijia;XU Ting;ZHOU You;YING Shuaiyu;YE Siting;DU Junying;FANG Junfan;FANG Jianqiao;GAO Hong;LIANG Yi(Department of Neurobiology and Acupuncture Research,the Third Clinical Medical College,Zhejiang Chinese Medical University,Key Laboratory of Acupuncture and Neurology of Zhejiang Province,Hangzhou 310053,China;Department of Acupuncture and Moxibustion,the Third Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学第三临床医学院针灸神经生物学实验室浙江省针灸神经病学重点实验室,杭州310053 [2]浙江中医药大学附属第三医院针灸科,杭州310053

出　　处：《中国疼痛医学杂志》2022年第5期331-339,共9页Chinese Journal of Pain Medicine

基　　金：浙江省自然科学基金(LY19H270010);国家级大学生创新创业训练集项目(201910344056)。

摘　　要：目的:动态检测完全弗氏佐剂(complete Freund’s adjuvant,CFA)诱导的炎性痛大鼠健患侧足跖厚度、痛行为和背根神经节(dorsal ganglia root,DRG)中P2X3受体(purinergic P2X3 receptor,P2X3R)、辣椒素受体(transient receptor potential vanilloid type 1,TRPV1)蛋白表达,探索炎性痛不同时期痛行为差异及其可能机制。方法:采用大鼠左侧足跖注射CFA建立炎性痛模型。将大鼠按随机数字表法分为对照组(control,Con group)和炎性痛组(CFA group)。分别检测基线(baseline,BL)和造模后1、3、7、14、28、42天时大鼠双侧足跖厚度、热缩足反射潜伏期(thermal withdrawal latency,TWL)和机械刺激缩足反射阈值(mechanical withdrawal threshold,MWT);Western bolt检测健(contralateral,Contra)患(ipsilateral,Ipsi)侧L_(4)-L_(6)DRG中P2X3R、TRPV1蛋白表达。结果:CFA大鼠患侧足跖厚度于造模后各时间点均显著高于Con-Ipsi组和CFA-Contra组。CFA大鼠患侧TWL仅于造模后1、3、7、14天时显著低于同期Con-Ipsi组,于造模后所有时间点均显著低于CFA-Contra组;CFA大鼠健侧TWL各时间点与Con-Contra相比均无差异。CFA大鼠患侧MWT于造模后所有时间点均显著低于同期Con-Ipsi组,于造模后1、3、7、14、28天时显著低于同期CFA-Contra组;与同期Con-Ipsi和Con-Contra组比,CFA大鼠健侧MWT于造模后28、42天时出现明显降低。CFA大鼠患侧DRG中P2X3R、TRPV1蛋白表达于造模后1、14、28、42天时均显著高于Con-Ipsi和Con-Contra组;CFA大鼠健侧DRG中P2X3R蛋白表达于造模后28、42天时显著多于Con-Contra组,而健侧DRG中TRPV1蛋白表达无差异。结论:CFA致炎性痛大鼠患侧足跖热痛觉过敏和机械痛觉异常持续存在,炎性痛后期可诱发出健侧足跖机械痛觉异常;P2X3R和TRPV1可能参与CFA大鼠早期和晚期外周痛敏化发生和维持,健侧背根神经节内P2X3R表达增加可能参与机械镜像痛的产生。Objective:To explore the differences in pain behavior at diverse phases of inflammatory pain and its underlying mechanism,by detecting the dynamic changes in ipsilateral and contralateral paw thickness and pain behavior as well as P2X3 and TRPV1 receptor protein expression in the dorsal root ganglion of CFA-induced inflammatory pain rats.Methods:Inflammatory pain model was established by injecting 0.1 ml of CFA into the plantar surface of rat’s left hindpaw.Rats were randomly divided into control group(Con)and CFA-induced inflammatory pain group(CFA).The changes of ipsilateral and contralateral paw thickness,thermal withdrawal latency(TWL)and mechanical withdrawal threshold(MWT)of rats were detected at baseline,1d,3d,7d,14d,28d and 42 d after modeling;protein expression of P2X3R and TRPV1 in ipsilateral and contralateral L_(4)-L_(6)DRG were detected by Western blot assay.Results:Compared with the Con-Ipsi group and CFA-Contra group at the same timepoint,the paw thickness in the ipsilateral side of CFA rats significantly increased at all timepoints.Ipsilateral TWL of CFA rats were significantly lower than those of Con-Ipsi rats at 1d,3d,7d and 14d after modeling and lower than those of CFA-contra rats at all timepoints observed.There is no significant difference in contralateral TWL between the CFA-Contra group and the Con-Contra group at all time points.Ipsilateral MWT of CFA rats was remarkedly lower than those of the Con-Ipsi rats at all timepoint observed and lower than those of CFA-contra rats at 1d,3d,7d,14d and 28d after modeling.Compared with the Con-Ipsi and Con-Contra group at the same timepoint,contralateral MWT significantly decreased.Both P2X3R and TRPV1 protein expression in ipsilateral DRGs of CFA rats were higher than those of the Con-Ipsi and Con-Contra group at all timepoints.P2X3R protein expression in contralateral DRGs of CFA rats were significantly higher than those in Con-Contra group at 28d and 42 d post-modeling,but TRPV1 protein remained unchanged.Conclusion:Thermal hyperalgesia and mec

关 键 词：炎性痛 热痛觉过敏 机械痛觉异常 背根神经节 P2X3R TRPV1 

分 类 号：R402[医药卫生—临床医学]