STEMI患者外周血p⁃p38MAPK、p⁃JNK表达与细胞凋亡及近期预后的关系  

Relationship between the expression of p⁃p38MAPK and p⁃JNK in peripheral blood of STEMI patients and apoptosis,short⁃term prognosis

作　　者：王发波 叶绵赋 李莉 许志魏 WANG Fabo;YE Mianfu;LI li;XU Zhiwei(Emergency Department,Tunchang County People's Hospital,Tunchang,Hainan,China,571600)

机构地区：[1]屯昌县人民医院急诊科,海南屯昌571600

出　　处：《分子诊断与治疗杂志》2022年第5期789-792,797,共5页Journal of Molecular Diagnostics and Therapy

基　　金：海南省卫生健康行业科研项目(21A200390)。

摘　　要：目的研究ST段抬高型心肌梗死(STEMI)患者外周血磷酸化p38丝裂原活化蛋白激酶(p⁃p38MAPK)、磷酸化c⁃Jun氨基末端激酶(p⁃JNK)表达与细胞凋亡及近期预后的关系。方法选择2019年5月至2021年6月期间在屯昌县人民医院急诊科成功行静脉溶栓的STEMI患者作为STEMI组、体检的健康志愿者作为对照组。检测外周血单个核细胞(PBMCs)中p⁃p38MAPK、p⁃JNK的表达水平,血清中Caspase⁃3、Caspase⁃12、sTWEAK含量,随访STEMI患者静脉溶栓成功后6个月的预后情况。结果STEMI组患者PBMCs中p⁃p38MAPK、p⁃JNK的表达水平及血清中Caspase⁃3、Caspase⁃12、sTWEAK含量均显著高于对照组,差异有统计学意义(P<0.05)。STEMI组患者p⁃p38MAPK、p⁃JNK与Caspase⁃3、Caspase⁃12、sTWEAK呈正相关(P<0.05)。STEMI组中多支病变、Gensini积分>40分、近期预后不良患者PBMCs中p⁃p38MAPK、p⁃JNK的表达水平均显著高于单支病变、Gensini积分0⁃40分、近期预后良好患者,差异有统计学意义(P<0.05)。p⁃p38MAPK、p⁃JNK表达水平对STEMI患者的近期预后具有预测价值(P<0.05)。结论STEMI患者外周血p38MAPK、JNK的过度磷酸化与病情加重、细胞凋亡激活、近期预后不良有关。Objective To study the relationship between the expression of phosphorylated p38 mi⁃togen activated protein kinase(p⁃p38MAPK)and phosphorylated c⁃Jun N⁃terminal kinase(p⁃JNK)in periph⁃eral blood and apoptosis,short⁃term prognosis of patients with ST segment elevation myocardial infarction(STEMI).Methods STEMI patients who successfully underwent thrombolytic therapy in our hospital from May 2019 to June 2021 were selected as the STEMI group and healthy volunteers who underwent physical examination as the control group.The expression levels of p⁃p38MAPK and p⁃JNK in peripheral blood mono⁃nuclear cells(PBMCs)and the contents of Caspase⁃3,Caspase⁃12 and sTWEAK in serum were measured.The prognosis of STEMI patients within 6 months after thrombolytic therapy was followed up.Results The expression levels of p⁃p38MAPK and p⁃JNK in PBMCs and the contents of Caspase⁃3,Caspase⁃12 and sTWEAK in serum of the STEMI group were higher than those of the control group(P<0.05).p⁃p38MAPK and p⁃JNK were positively correlated with Caspase⁃3,Caspase⁃12 and sTEAK in the STEMI group.In the STEMI group,the expression levels of⁃p38MAPK and p⁃JNK in PBMCs of patients with multivessel lesions,Gensini score>40 and poor short⁃term prognosis were higher than those of patients with singlevessel lesions,Gensini score 0⁃40 and good short⁃term prognosis(P<0.05).The expression levels of p⁃p38MAPK and p⁃JNK have predictive value on the short⁃term prognosis of STEMI patients(P<0.05).Conclusion Hyperphosphorylation of p38MAPK and JNK in peripheral blood of patients with STEMI is related to disease aggravation,activation of apoptosis and poor short⁃term prognosis.

关 键 词：ST段抬高型心肌梗死 P38MAPK JNK 

分 类 号：R542.22[医药卫生—心血管疾病]