异常早幼粒细胞Auer小体发生率的影响因素分析  

Analysis on the factors affecting the incidence of abnormal promyelocytic Auer bodies

作　　者：张景逍 张金彪[1] 张晗钰 翟文燕 王晓静 ZHANG Jingxiao;ZHANG Jinbiao;ZHANG Hanyu;ZHAI Wenyan;WANG xiaojing(Department of Clinical Laboratory,Cangzhou Hospital of Integrated TCM-WM·Heibei,Cangzhou,Hebei 061000,China)

机构地区：[1]河北省沧州中西医结合医院实验诊断科,河北沧州061000

出　　处：《检验医学与临床》2022年第12期1657-1659,1663,共4页Laboratory Medicine and Clinic

摘　　要：目的探讨急性早幼粒细胞白血病(APL)患者异常早幼粒细胞Auer小体发生率的影响因素。方法收集2018年5月至2020年5月该院确诊的38例APL患者,计数出现Auer小体的异常早幼粒细胞的比例作为Auer小体发生率。采用秩和检验比较不同PML/RARα融合基因类型、CD56与HLA-DR表达、性别、年龄患者Auer小体发生率的差异;采用Spearman相关分析异常早幼粒细胞各项流式表达率(CD13、CD33、CD117、CD38、CD64)及初诊白细胞计数(WBC)、血红蛋白(Hb)、血小板计数(PLT)和融合基因剪接体相对表达量(PML/ABL)与Auer小体发生率之间的相关性,采用简单线性回归对Auer小体发生率的影响因素进行单因素分析。结果CD56阴性组的Auer小体发生率高于阳性组,PML/RARα融合基长链型(L型)Auer小体发生率高于短链型(S型),差异均有统计学意义(P<0.05);初诊WBC与Auer小体发生率呈负相关(r=-0.376,P<0.05)。结论CD56的表达、PML/RARα融合基因类型与患者初诊WBC均可影响APL患者Auer小体的发生率。Objective To investigate the factors affecting the incidence of abnormal promyelocytic Auer bodies in patients with acute promyelocytic leukemia(APL).Methods A total of 38 APL patients diagnosed in this hospital from May 2018 to May 2020 were collected,and the proportion of abnormal promyelocytic granulocytes with Auer bodies was counted as the incidence of Auer bodies.Rank sum test was used to compare the incidence of Auer bodies in patients with different PML/RARαfusion gene types,CD56 and HLA-DR expression,gender and age.The correlation between flow cytometry expression rates of abnormal promyelocytic granulocytes(CD13,CD33,CD117,CD38,CD64),white blood cell count(WBC),hemoglobin(Hb),platelet count(PLT),fusion gene spliceosomal relative expression(PML/ABL)and Auer bodies were analyzed by Spearman correlation.Simple linear regression was used to analyze the factors influence the incidence of Auer bodies.Results The incidence of Auer bodies in CD56 negative group was higher than that in positive group,for the PML/RARαfusion gene,the incidence of Auer bodies in long chain type(L type)was higher than that in short chain type(S type),the differences were statistically significant(P<0.05).The incidence of Auer bodies was negatively correlated with newly diagnosed WBC(r=-0.376,P<0.05).Conclusion The expression of CD56,PML/RARαfusion gene and WBC in patients with newly diagnosed APL may influence the incidence of Auer bodies.

关 键 词：急性早幼粒细胞白血病 AUER小体 PML/RARΑ融合基因 

分 类 号：R446.113[医药卫生—诊断学]