PAR-2、COX-2在嗜酸性慢性鼻-鼻窦炎鼻息肉患者组织中的表达及其临床意义  

The expression and clinical significance of PAR-2 and COX-2 in the tissues of patients with eosinophilic chronic rhinosinusitis with nasal polyps

作　　者：李俊娟 熊向菁 王青海 谢添慧 LI Junjuan;XIONG Xiangjing;WANG Qinghai;XIE Tianhui(Department of Otolaryngology,the First People’s Hospital of Xining,Xining 810001,China)

机构地区：[1]西宁市第一人民医院耳鼻喉科,青海西宁810001

出　　处：《现代医学》2022年第3期336-342,共7页Modern Medical Journal

摘　　要：目的:探讨蛋白酶激活受体2(PAR-2)及环氧化酶-2(COX-2)在慢性鼻-鼻窦炎鼻息肉(CRSwNP)患者组织中的表达水平及关系,并分析其临床意义。方法:选取2017年11月至2020年11月本院收治的CRSwNP患者133例,将其分为嗜酸性慢性鼻窦炎鼻息肉(ECRSwNP)组70例、非嗜酸性慢性鼻窦炎鼻息肉(non-ECRSwNP)组63例,另同期选取因行鼻中隔偏曲矫正术或下鼻甲肥大切除术患者70例为对照组。取其鼻黏膜组织,对3组标本进行苏木精-伊红(HE)染色;分别采用实时荧光定量PCR(qRT-PCR)法、免疫组织化学法测定组织中PAR-2、COX-2 mRNA水平及其蛋白表达情况;采用Pearson相关系数法分析PAR-2、COX-2 mRNA水平与Lund-Mackay评分相关性。结果:ECRSwNP组组织上皮下间质可见大量双核红染的嗜酸性粒细胞,non-ECRSwN组组织嗜酸性粒细胞较少;ECRSwNP组嗜酸性粒细胞百分比显著高于non-ECRSwN组(P<0.05)。PAR-2蛋白定位于组织黏膜上皮细胞及下腺体细胞胞浆及胞膜,COX-2蛋白主要定位于组织黏膜上皮细胞胞浆及胞膜,均呈棕黄色颗粒;ECRSwNP组、non-ECRSwN组Lund-Mackay评分、PAR-2、COX-2 mRNA水平及蛋白表达阳性率均显著高于对照组,ECRSwNP组Lund-Mackay评分、PAR-2、COX-2 mRNA水平及蛋白表达阳性率均显著高于non-ECRSwN组(P<0.05);Pearson相关系数法结果显示ECRSwNP患者组织标本中PAR-2、COX-2 mRNA水平与Lund-Mackay评分均呈正相关,且PAR-2 mRNA与COX-2 mRNA水平呈正相关(P<0.05)。结论:PAR-2、COX-2在ECRSwNP患者鼻窦组织中异常高表达,且可一定程度反映病情严重程度,可作为病情判断及疗效观察的潜在指标。Objective:To investigate the expression levels and relationship of protease-activated receptor(PAR-2)and cyclooxygenase-2(COX-2)in the tissues of patients with chronic rhinosinusitis with nasal polyps(CRSwNP),and analyze their clinical significance.Methods:One hundred and thirty-three patients with CRSwNP admitted to our hospital from November 2017 to November 2020 were selected,and they were divided into eosinophilic chronic sinusitis with nasal polyps(ECRSwNP)group(70 cases),non-eosinophilic chronic sinusitis with nasal polyps(non-ECRSwNP)group(63 cases),another seventy cases of patients who underwent nasal septum correction or inferior turbinate hypertrophy were selected as the control group.The nasal mucosa tissue was taken and hematoxylin-eosin(HE)staining was performed on the three groups of specimens;real-time fluorescent quantitative PCR(qRT-PCR)method and immunohistochemistry method were used to determine the PAR-2 and COX-2 mRNA levels and protein expression in tissues;Pearson correlation coefficient method was used to analyze the correlation between PAR-2,COX-2 mRNA levels and Lund-Mackay score.Results:There were a large number of binuclear red-stained eosinophils in the subepithelial mesenchyme of the ECRSwNP group,while fewer eosinophils in the non-ECRSwN group;the percentage of eosinophils in ECRSwNP group was significantly higher than that in non-ECRSwN group(P<0.05).PAR-2 protein was located in the cytoplasm and membrane of tissue mucosal epithelial cells and lower gland cells,while COX-2 protein was mainly located in the cytoplasm and membrane of tissue mucosal epithelial cells,both of which were brown particles;the Lund-Mackay score,PAR-2,COX-2 mRNA levels and protein expression positive rate in the ECRSwNP group and non-ECRSwN group were significantly higher than those in the control group.The Lund-Mackay score,PAR-2,COX-2 mRNA levels and protein expression positive rate in ECRSwNP group were significantly higher than those in non-ECRSwN group(P<0.05);the Pearson correlation coefficient method

关 键 词：蛋白酶激活受体 环氧化酶-2 慢性鼻-鼻窦炎鼻息肉 临床意义 

分 类 号：R765.2[医药卫生—耳鼻咽喉科] R765.41[医药卫生—临床医学]