植物UGTs的功能、应用与分子改造研究进展  

Research progress on the function,application and molecular modification of plant UDP-glycosyltransferase

作　　者：文超 欧阳文虹 黄伟[2] 聂嘉兴 邓文丽 张训华 郑丽娟 WEN Chao;OUYANG Wenhong;HUANG Wei;NIE Jiaxing;DENG Wenli;ZHANG Xunhua;ZHENG Lijuan(Department of Medicine,Jinggangshan University,Ji’an 343009,Jiangxi,China;College of Medicine,Wuhan University,Wuhan 430072,Hubei,China;Testing Center of Jiangxi Hukeng Tungsten Industry Co.,Ltd,Ji’an 343205,Jiangxi,China)

机构地区：[1]井冈山大学医学部,江西吉安343009 [2]武汉大学药学院,湖北武汉430062 [3]江西浒坑钨业有限公司测试中心,江西吉安343205

出　　处：《生物资源》2022年第2期122-129,共8页Biotic Resources

基　　金：国家自然科学基金(32160059);江西省教育厅科技计划项目(GJJ201035);大学生创新创业训练项目(202010419029)。

摘　　要：植物尿苷二磷酸糖基转移酶(uridine diphosphate,UGTs),在次生代谢产物生物合成、激素平衡、免疫防御、脱毒反应等生理过程中发挥重要作用。随着酶功能表征和结构研究的不断深入,具有催化高效性、区域/立体选择性和底物宽容性的植物UGTs可用于天然/非天然糖苷的酶法直接合成及异源生物合成,植物UGTs酶分子理性改造与定向进化的成功例子也在不断增加。围绕生物学功能、糖基化应用与酶分子改造三个方面,对近几年来植物UGTs的相关研究进行系统综述。同时,对植物UGTs基因的准确预测与筛选、系统功能分析及酶分子理性设计等方面的发展趋势进行展望。Uridine diphosphate-glycosyltransferases(UGTs)play important roles in physiological processes,such as secondary metabolite biosynthesis,hormone homeostasis,immune defense,and detoxification reactions.With the deepening of the study of enzyme characterization and structure,plant UGTs with catalytic efficiency,regiospecificity/stereoselectivity and substrate promiscuity can be used for direct enzymatic synthesis or heterologous biosynthesis of natural/non-natural glycosides,and the successful examples of rational modification and directed evolution of plant UGTs are continuously increasing.This systematic review of relevant studies on plant UGTs is presented around three aspects:biological function,glycosylation application and enzyme modification.At the same time,the development trend of accurate prediction and screening of plant UGTs genes,systematic functional analysis and rational design of enzyme molecules is prospected.

关 键 词：尿苷二磷酸糖基转移酶 糖基化 植物 次生代谢产物 

分 类 号：Q555.4[生物学—生物化学]