中山地区CD36阴性血小板供者库建立的探讨  

Establishment of CD36 negative platelet donor bank in Zhongshan area

作　　者：吴泳伦 孙爱农 蒲菲 李乔 方育如 陈前英 廖艳婷 王红梅[2,3] 陈晔洲 段生宝 WU Yonglun;SUN Ainong;PU fei;LI Qiao;FANG Yuru;CHEN Qianying;LIAO Yanting;WANG Hongmei;CHEN Yezhou;DUAN Shengbao(Zhongshan Blood Center,Zhongshan 528400,China;Suzhou Institute of Biomedical Engineering and Technology;Jihua Laboratory)

机构地区：[1]中山市中心血站,广东中山528400 [2]中国科学院苏州生物医学工程技术研究所 [3]季华实验室

出　　处：《中国输血杂志》2022年第5期558-561,共4页Chinese Journal of Blood Transfusion

基　　金：中山市社会公益与基础研究项目(2020B1065);季华实验室项目(X190171TD190)。

摘　　要：目的 了解中山市无偿献血人群中CD36缺失频率及其CD36抗原阴性者基因突变情况,探讨建立当地CD36阴性血小板供者库建立的可能性。方法 对1 654名无偿献血者,采用酶免试验法进行血小板CD36抗原检测,部分阴性者以流式细胞仪技术进行确认和分型,同时进行基因检测。结果 血小板CD36抗原阴性27名,占1.6%(27/1 654);阴性者中,男性18名占1.6%(18/1 149),女性9名占1.8%(9/505),CD36抗原缺失者男性和女性比较,P>0.05,差异无显著性。随机对其中CD36阴性的15例标本进行分型和基因测序,结果Ⅰ型缺失1例占6.7%(1/15),Ⅱ型缺失14例占93.3%(14/15),8例检出外显子3~外显子14发生基因突变。结论 本地区血小板CD36抗原缺失频率与中国其它南部地区相当,CD36阴性献血者血小板的供者库的建立,将有助于提高血小板输血有效性。Objective To investigate the frequency of CD36 deletion and gene mutation in voluntary blood donors of Zhongshan city, and to explore the possibility of establishing local CD36 negative platelet donor bank.Methods Platelet CD36 antigen was detected by ELISA in 1 654 voluntary blood donors.Some of the negative samples were confirmed by flow cytometry, and genotyping was also performed.Results Platelet CD36 antigen was negative in 27 cases, accounting for 1.6%(27/1654),among which 1.6%(18/1149) were males and 1.8%(9/505) were females.No significant difference was noticed between males and females in CD36 antigen deletion cases(P>0.05).Fifteen CD36 negative samples were randomly selected, genotyped and sequenced, with type I deletion in 1 case[ 6.7%(1/15)],type Ⅱ deletion in 14 cases[ 93.3%(14/15)],and gene mutation in exon 3-14 detected in 8 cases.Conclusion The frequency of platelet CD36 antigen deletion in Zhongshan is comparable to that in other southern regions of China.The establishment of CD36 negative platelet donor bank is conductive to improve the effectiveness of platelet transfusion.

关 键 词：CD36抗原 血小板 献血者 供者库 

分 类 号：R331.143[医药卫生—人体生理学]